CD147 抗体

CD147 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CD147.

CD147：预测可启用细胞间粘附介体活性；信号受体活性；和病毒受体活性。参与神经视网膜发育；感光细胞维护；和精子发生。位于几种细胞成分中，包括顶体膜；感光器内段；和光感受器外段。以多种结构表达，包括消化系统；脑;早孕;生殖系统;和感觉器官。与人 BSG 同源（basigin（Ok 血型））。[由基因组资源联盟提供，2022 年 4 月]

Free

P18572

Predicted band size: 42 kDa;Observed band size: 38-58 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102621

Immunology

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Mouse

WB: 1:500-1:1000; IHC: 1:50-1:100; IP: 1:20; FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela(lane 2(20ug) , C2C12(lane 3(20ug) using CD147 Antibody (HY-P80596) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using CD147 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using CD147 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, FC, IP

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Mouse CD147.The exact sequence is proprietary to MCE.