Elongation factor 2 抗体 (YA962)

目录号: HY-P81272: Data Sheet SDS COA 抗体使用指南
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Elongation factor 2 Antibody (YA962) 是一个小鼠来源、无偶联标记、抗 Elongation factor 2 的 IgG2b 单克隆抗体。

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规格	价格	是否有货
10 μL	￥470	In-stock
50 μL	￥1220	In-stock
100 μL	￥2000	In-stock
250 μL		询价

or 大包装询价

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Elongation factor 2 Antibody (YA962) 相关产品:

WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

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验证图片
背景信息
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描述

Elongation factor 2 Antibody (YA962) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Elongation factor 2.

宿主

Mouse

克隆性

Monoclonal

分子量

Predicted band size: 95 kDa;

Observed band size: 95 kDa

请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

反应种属

Human

蛋白数据库

P13639

基因 ID

1938 [NCBI]

免疫原

Purified recombinant human eEF2 protein fragments expressed in E.coli.

应用 & 推荐
稀释比例

应用	稀释比
WB WB: 蛋白质免疫印迹	1:500-1:1000
ICC/IF ICC/IF: 细胞免疫荧光	1:50-1:200
ChIP ChIP: 染色质免疫沉淀	1:20

敏感性	Endogenous	纯度	Affinity Purified
偶联	Non-conjugated	修饰	Unmodified
同型	IgG2b

性状

液体

组分

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

ICC

Immunocytochemistry analysis of Hela cells labeling Elongation factor 2 with Elongation factor 2 Antibody(HY-P81272) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Elongation factor 2 Antibody(HY-P81272) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of NIH/3T3 cells labeling Elongation factor 2 with Elongation factor 2 Antibody(HY-P81272) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Elongation factor 2 Antibody(HY-P81272) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

RRID

AB_3103335

反应种属数据库

Entrez Gene: 1938 Human

SwissProt: P13639 Human

OMIM: 609306 Human

研究领域

Epigenetics and Nuclear Signaling

中文名

Elongation factor 2 抗体 (YA962)

同用名

EEF2; EF2; Elongation factor 2; EF-2

文件资料

Select Batch:

Data Sheet (442 KB) SDS (251 KB)

COA (193 KB)

抗体使用指南 (1083 KB)

Elongation factor 2 Antibody (YA962) 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

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上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Elongation factor 2 抗体 (YA962)

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