FER 抗体 (YA3830)

FER Antibody (YA3830) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FER.

FER 是一种酪氨酸蛋白激酶 (tyrosine-protein kinase)，在生长因子细胞表面受体下游发挥作用，参与调控肌动蛋白细胞骨架 (actin cytoskeleton)、微管组装 (microtubule assembly)、板状伪足形成 (lamellipodia formation)、细胞粘附 (cell adhesion)、细胞迁移 (cell migration) 和趋化性 (chemotaxis)。它在 EGFR、KIT、PDGFRA 和 PDGFRB 下游发挥作用，并通过 EGFR 促进 NF-kappa-B 的激活和细胞增殖。FER 可能在有丝分裂细胞周期 (mitotic cell cycle) 调控中发挥作用，并参与胰岛素受体信号通路 (insulin receptor signaling pathway) 和磷脂酰肌醇 3-激酶 (phosphatidylinositol 3-kinase) 的激活。此外，FER 在激活的 FCER1 受体下游发挥作用，参与肥大细胞 (mast cells) 中 FCER1 介导的信号传导，并调控肥大细胞脱颗粒 (mast cell degranulation)。FER 还参与细菌脂多糖 (LPS) 诱导的白细胞募集 (leukocyte recruitment) 和渗出 (diapedesis)。在突触组织中，FER 参与突触小泡运输 (trafficking of synaptic vesicles)、兴奋性突触后电流 (excitatory postsynaptic currents) 的生成以及神经元间突触传递 (neuron-neuron synaptic transmission)。FER 在脑损伤后的神经元细胞死亡 (neuronal cell death) 中发挥作用。FER 可以磷酸化 CTTN、CTNND1、PTK2/FAK1、GAB1、PECAM1 和 PTPN11，可能磷酸化 JUP 和 PTPN1，并能磷酸化 STAT3，但其生物学相关性取决于细胞类型和刺激。

P16591

Predicted band size: 95 kDa; Observed band size: 95 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal Antibody

Mouse

Human, Mouse

WB: 1:500-1:2000; IHC: 1:200-1:1000; IF: 1:200-1:1000; ELISA: 1:10000

• 
  Western blot analysis of extracts from NIH3T3 (lane 2(20μg) and NIH3T3 (lane 3(40μg), A549 (lane 4(20μg) and A549 (lane 5(40μg) using FER (HY-P84133) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IHC-P, ICC/IF, ELISA

液体

Supplied in ascitic fluid containing 0.03% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human FER aa 280-474.