MLKL 抗体 (YA289)

MLKL Antibody (YA289) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MLKL.

MLKL 是一种假激酶，在 TNF 诱导的坏死性凋亡中发挥着关键作用，坏死性凋亡是一种以质膜损伤和钙内流为特征的程序性细胞死亡过程。尽管缺乏内在蛋白激酶活性，MLKL 在被 RIPK3 磷酸化后被激活。这种激活导致同源三聚化、质膜定位和坏死性凋亡的执行。此外，为了应对正粘病毒感染，MLKL 可以经历由 ZBP1 激活引发的核坏死性凋亡，导致核膜破坏并将细胞 DNA 释放到胞质溶胶中。MLKL 的坏死性凋亡功能通过其与高度磷酸化的肌醇磷酸盐（例如肌醇六磷酸 (InsP6)）的结合而得到促进。这种结合不仅介导 N 末端自动抑制区域的释放，而且还需要 RIPK3 依赖性磷酸化才能完全激活。Necrosulfonamide 是一种特异性坏死性凋亡抑制剂，以 Cys-86 为靶点，有效抑制 MLKL 功能。

Free

Q8NB16

Predicted band size: 54 kDa； Observed band size: 54 kDa

Protein A affinity purified.

Cytoplasm, Cell membrane, Golgi apparatus.

Non-conjugated

Unmodified

AB_3102361

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1,000 ;IHC-P: 1:50

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) , THP-1(lane 3(20μg) and U937(lane 4(20μg) using MLKL(HY-P80227) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using MLKL Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80227, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using MLKL Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80227, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human MLKL.AA range:333-471.