NADPH Oxidase 4 抗体

NADPH Oxidase 4 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to NADPH Oxidase 4.

NADPH Oxidase 4：该基因编码 NOX 酶家族的一个成员，其功能是 NADPH 氧化酶复合物的催化亚基。编码的蛋白质定位于非吞噬细胞，在那里它充当氧传感器并催化分子氧还原为各种活性氧 (ROS)。这种蛋白质产生的 ROS 与许多生物学功能有关，包括信号转导、细胞分化和肿瘤细胞生长。已在 11 号染色体的另一臂上鉴定出一个假基因。可变剪接导致多个转录变体。[由 RefSeq 提供，2009 年 1 月]

Free

Q9NPH5

Predicted band size: 67 kDa; Observed band size: 67 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102700

Cardiovascular

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; IP: 1:20

• 
  Western blot analysis of extracts from Hela using NADPH Oxidase 4 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded human Lung squamous cell carcinoma tissue using NADPH Oxidase 4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
• 
  Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using NADPH Oxidase 4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
• 
  Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using NADPH Oxidase 4 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
• 
  Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using NADPH Oxidase 4 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

WB, IHC-P, ICC/IF, IP

液体

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human NOX4.The exact sequence is proprietary to MCE.