SATB2 抗体 (YA842)

SATB2 Antibody (YA842) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SATB2.

SATB2 是一种能够结合 DNA 的转录因子，主要作用于核基质或支架相关区域（MARs），识别双链 DNA 的糖-磷酸结构。它通过结合 DNA 的 MARs 并诱导局部染色质环重塑，调控核基因表达。SATB2 可作为染色质重塑酶的锚定位点，直接招募辅阻遏物（如 HDACs）或辅激活物（如 HATs）至启动子和增强子区域。在上层神经元（UL1）特异性遗传程序的启动以及深层神经元（DL）和 UL2 特异性基因的失活中起关键作用，可能通过调控 BCL11B 表达实现。此外，SATB2 是 Ctip2 的阻遏物，也是发育中大脑皮层皮质-皮质连接的调控决定因子，并在腭形成中发挥重要作用。它还是调控骨骼发育和成骨细胞分化的转录网络中的分子节点。

Q9UPW6

Predicted band size: 83 kDa; Observed band size: 83 kDa

Non-conjugated

Unmodified

AB_3102773

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; ICC: 1:50-1:100; IF-Tissue: 1:50-1:100; IHC-P: 1:50-1:200; FC: 1:50-1:100

• 
  Western blot analysis of extracts from THP-1(lane 2(20μg) , SaoS-2(lane 3(20μg) and C6(lane 4(20ug) using SATB2(HY-P80958 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Saos-2 cells labeling SATB2 with SATB2 Antibody (HY-P80958) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with SATB2 Antibody (HY-P80958) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling SATB2 with SATB2 Antibody (HY-P80958) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with SATB2 Antibody (HY-P80958)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded mouse brain tissue using SATB2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse brain tissue using SATB2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human SATB2.AA range: 238-287.