SNAI1 抗体

SNAI1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SNAI1.

SNAI1 是一种参与上皮间质转化 (EMT) 诱导、胚胎中胚层形成与维持、生长停滞、细胞存活及迁移的蛋白质。它能够结合 E-cadherin/CDH1 基因启动子的 3 个 E-box 以及 CLDN7 和 KRT8 的启动子区域，并通过招募组蛋白去甲基化酶 KDM1A 至这些启动子，降低二甲基化 H3K4 水平，从而抑制转录。其 N 端 SNAG 结构域与组蛋白 H3 竞争结合 KDM1A 和 RCOR1 形成的组蛋白去甲基化酶复合物上的相同位点，进而抑制组蛋白 H3 在 Lys-4 位点的去甲基化 (体外实验)。在 EMT 过程中，SNAI1 与 LOXL2 共同参与负调控着丝粒周围异染色质的转录 (By similar: SNAI1 招募 LOXL2 至着丝粒周围区域氧化组蛋白 H3 并抑制转录，导致异染色质组分 CBX5/HP1A 释放，促进染色质重组和间质特征获得)。此外，SNAI1 还能与 EGR1 和 SP1 结合，介导佛波酯 (TPA) 诱导的 CDKN2B 上调，可能通过结合 CDKN2B 启动子区域的 5'-TCACA-3' 序列实现，并可能单独激活 CDKN2B 启动子。

O95863

Predicted band size: 29 kDa; Observed band size: 29 kDa

affinity purified

Nucleus. Cytoplasm. Note=Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

Non-conjugated

Unmodified

AB_3103067

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; ELISA: 1:10000

• 
  Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), NIH/3T3 (lane 3(40μg), using SNAI1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using SNAI1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using SNAI1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, ELISA

液体

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

The antiserum was produced against synthesized peptide derived from human SNAI1.