Survivin/BIRC5 抗体 (YA045)

Survivin/BIRC5 Antibody (YA045) is a non-conjugated and Rabbit origined monoclonal antibody about 16 kDa, targeting to Survivin. It can be used for WB,ICC/IF,IHC-P,FC assays with tag free, in the background of Human, Mouse.

BIRC5是一种多功能蛋白，在促进细胞增殖和防止细胞凋亡方面发挥双重作用，凸显其多方面的功能。作为染色体通道蛋白复合物 (CPC) 的重要组成部分，BIRC5 对于有丝分裂和胞质分裂过程中的染色体排列和分离至关重要。它协调 CPC 运动，引导其从中期的内部着丝粒定位到胞质分裂期间的中体，并通过与聚合微管结合来促进中央纺锤体组织。BIRC5 错综复杂地参与了早期有丝分裂期间将 CPC 招募到着丝粒的过程，并与在“Thr-3”(H3pT3) 处磷酸化的组蛋白 H3 结合。它与 RAN 协同发挥作用，有助于有丝分裂纺锤体的形成，充当将 RAN 效应分子 TPX2 传递到微管的支架。除了其有丝分裂功能之外，BIRC5 还可以对抗 G2/M 期默认的细胞凋亡诱导，其乙酰化形式可以抑制靶基因启动子的 STAT3 反式激活。此外，它还是 CASP3 和 CASP7 的抑制剂，对于维持线粒体完整性和功能至关重要。BIRC5 以 CPC 结合状态的单体存在，可有效保护细胞免于凋亡，而其二聚体形式则增强微管蛋白的稳定性。BIRC5 参与与各种蛋白质（例如组蛋白 H3、RAN、微管蛋白、XIAP/BIRC4 和 DIABLO/SMAC）相互作用的复杂网络，强调了其在细胞过程中的关键调节功能。

Free

O15392

Predicted band size: 16 kDa; Observed band size: 16 kDa

Protein A affinity purified.

Cytoplasm, Nucleus, Chromosome, Midbody.

Non-conjugated

Unmodified

AB_3102450

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:500-1:1,000 ;ICC/IF: 1:50-1:200 ;IHC-P: 1:50-1:500 ;FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) , Jurkat (lane 3(20μg) , RAW264.7 (lane 4(20μg),using Survivin Antibody (HY-P80352). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using Survivin Antibody (YA045). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80325, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using Survivin Antibody (YA045). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80325, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Survivin.The exact sequence is proprietary to MCE.