Topoisomerase I 抗体 (YA2237)

Topoisomerase I Antibody (YA2237) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Topoisomerase I.

TOP1 蛋白通过减轻 DNA 复制和转录过程中产生的 DNA 超螺旋和扭转张力，在细胞过程中发挥关键作用。这是通过 DNA 双链体一条链的瞬时切割和随后的重新连接来实现的。该酶通过酯交换作用在双链 DNA 的特定靶位点引入单链断裂，从而形成 DNA-(3'-磷酸酪氨酰)-酶中间体。该中间体排出 5'-OH DNA 链，使游离 DNA 链围绕相反链上完整的磷酸二酯键旋转，从而有效去除 DNA 超螺旋。在最后的再连接步骤中，DNA 5'-OH 攻击共价中间体，导致活性位点酪氨酸被驱逐并恢复 DNA 磷酸二酯主链。除了在 DNA 拓扑维持中的作用之外，TOP1 还参与选择性剪接的调节，其对内皮细胞中组织因子 (F3) 前 mRNA 的影响就是例证。此外，它通过修改 BMAL1 启动子上 ROR 响应元件 (RORE) 周围的染色质结构，有助于核心生物钟组件 BMAL1 的昼夜节律转录。

P11387

Predicted band size: 91 kDa; Observed band size: 91 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3104599

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200

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  Immunocytochemistry analysis of Hela cells labeling Topoisomerase I Antibody (HY-P82492) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Topoisomerase I Antibody (HY-P82492) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of MCF7 cells labeling Topoisomerase I Antibody (HY-P82492) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Topoisomerase I Antibody (HY-P82492) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Topoisomerase I Antibody (YA2237). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82492, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Topoisomerase I Antibody (YA2237). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82492, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-F, IHC-P, ICC/IF

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human Topoisomerase I