2. Biology Dictionary
  3. Equilibrium Dialysis

Equilibrium Dialysis平衡透析法

释义：

Two chambers are separated by a dialysis membrane. The molecular weight cut off (MWCO) of this membrane is chosen such that it will retain the receptor component of the sample (the element which will bind the ligand). A known concentration and volume of ligand is placed into one of the chambers. The ligand is small enough to pass freely through the membrane. A known concentration of receptor is then placed in the remaining chamber in an equivalent volume to that placed in the first chamber. As the ligand diffuses across the membrane some of it will bind to the receptor and some will remain free in solution. The higher the affinity of the interaction, the higher the concentration of ligand that will be bound at any time.

两个腔室通过透析膜分开。选择该膜的分子量截断 (MWCO) 使其能够保留样品中的受体组分 (即与配体结合的元素)。 已知浓度和体积的配体被放入其中一个腔室。该配体足够小，可以自由通过膜。然后，将已知浓度的受体放入剩余的腔室，其体积等于第一个腔室中放入的配体体积。当配体扩散通过膜时，一部分将与受体结合，另一部分将保持自由状态。相互作用的亲和力越高，结合的配体浓度就越高。

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