1. Metabolic Enzyme/Protease
  2. Phospholipase
  3. C12 NBD Sphingomyelin

C12 NBD Sphingomyelin 

目录号: HY-D1584 纯度: ≥99.0%
COA 产品使用指南

C12 NBD sphingomyelin 是一种具有活性的 sphingomyelin (HY-113498) 衍生物,带有荧光 C12 硝基苯并恶二唑 (C12 NBD)。C12 NBD sphingomyelin 可用作研究鞘磷脂酶代谢和转运的底物 (Ex=470 nm,Em=525 nm)。

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C12 NBD Sphingomyelin Chemical Structure

C12 NBD Sphingomyelin Chemical Structure

CAS No. : 254117-01-6

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100 μg ¥2400
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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

C12 NBD sphingomyelin is an active derivative of sphingomyelin (HY-113498) that is tagged with fluorescent C12 nitrobenzoxadiazole (C12 NBD). C12 NBD sphingomyelin can be used as a sphingomyelinase substrate for studying the metabolism and transport of sphingomyelins (Ex=470 nm, Em=525 nm)[1].

IC50 & Target

Sphingomyelinase[1]

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
Assay for Sphingolipid-Degrading Enzymes (EGCase Ⅱ, SCDase and SMase):
1. Incubate amounts of enzymes with 0.1 nM dye at 37 ℃ for indicated times under following conditions.
(1). 10 mM sodium acetate buffer (pH5.0) containing 0.2% Triton X-100 for EGCase.
(2) 25 mM sodium phosphate buffer (pH 6.0) containing 0.1% Triton X-100 for SCDase.
(3) 25 mM sodium phosphate buffer (pH 7.0) containing 0.2% Triton X-100 for SMase.
2. After incubation, the solvent is evaporated and the residue is dried, dissolved in 10 μL of chloroform/methanol (2:1) and analyzed by TLC using chloroform/methanol/0.02% CaCl2 (5:4:1, v/v) as the developing solvent.
3. Degradation products and remaining substrates are separated by TLC and quantified with a chromatoscanner (excitation 470 nm, emission 525 nm) for fluorescence-labeled substrates.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

825.03

Formula

C41H73N6O9P

CAS 号
性状

固体

颜色

Yellow to orange

Emission (Em)

525

Excitation (Ex)

485

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

纯度 & 产品资料

纯度: ≥99.0%

参考文献

C12 NBD Sphingomyelin 相关分类

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  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
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C12 NBD Sphingomyelin
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