2. Metabolic Enzyme/Protease Stem Cell/Wnt
  3. E1/E2/E3 Enzyme YAP
  4. DDO-4033

DDO-4033 是一种 SPOP 抑制剂 (IC50 = 16.9 μM, Kd = 15.1 μM)。DDO-4033 阻碍透明细胞肾细胞癌 (ccRCC) 细胞系的恶性迁移、侵袭和增殖。DDO-4033 破坏 SPOP 对底物 LATS1 的募集，抑制其多泛素化和随后的降解，上调 LATS1 的表达。DDO-4033 具有良好的抗肿瘤活性，可用于肾细胞癌研究。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

DDO-4033

DDO-4033 Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

DDO-4033 相关抗体

Top Publications Citing Use of Products

查看 YAP 亚型特异性产品:

查看所有亚型
YAP/TAZ YAP/TAZ-TEAD TEAD YAP1

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

DDO-4033 is a SPOP inhibitor (IC50 = 16.9 μM, Kd = 15.1 μM). DDO-4033 impairs the malignant migration, invasion, and proliferation of clear cell renal cell carcinoma (ccRCC) cell lines. DDO-4033 disrupts SPOP recruitment to its substrate LATS1, inhibits its polyubiquitination and subsequent degradation, and upregulates LATS1 expression. DDO-4033 has promising antitumor activity and is promising for renal cell carcinoma research[1].

体外研究
(In Vitro)

DDO-4033 对 786-O、A498、A549 和 HK-2 细胞表现出抗增殖活性，IC50 分别为 1.1、0.68、19.96 和 18.78 μM，但对 HepG2 细胞的抗增殖活性较弱，IC50 > 50 μM[1]。
DDO-4033 (0-3 μM，0-32 小时) 可抑制 SPOP 介导的 LATS1 泛素化和降解，下调 A498 细胞中 CTGF 和 CYR61 基因的 mRNA 水平[1]。
DDO-4033 (0-5 μM，24 小时) 可抑制 A498 细胞的集落形成并削弱细胞迁移能力[1]。
DDO-4033 在 MST、BLI 和 ITC 检测下表现出对 SPOP 的亲和力，Kd 值分别为 15.1、4.96、4.94 μM[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

DDO-4033 相关抗体:

Western Blot Analysis[1]

Cell Line: A498 cells
Concentration: 0 μM, 0.12 μM, 0.25 μM, 0.5 μM, 1 μM
Incubation Time: 0 h, 1 h, 2 h, 4 h,8 h, 12 h, 16 h, 32 h
Result: Inhibited LATS1 ubiquitination, prolongs the half-life of LATS1, and accumulates LATS1.

Cell Migration Assay [1]

Cell Line: A498 cells
Concentration: 0 μM, 0.5 μM, 1 μM
Incubation Time: 24 h
Result: Inhibited the colony formation and impaired the cell migratory capacity.
体内研究
(In Vivo)

DDO-4033 (10-50 mg/kg，肿瘤内 (i.t.) 和肿瘤周围 (PTI)，每天一次， 3 周) 在 A498-Luc 异种移植 BALB/c 裸鼠模型中可抑制肿瘤生长，上调 LATS1 并激活 Hippo 通路[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: A498-Luc (1 × 107 cells containing 25% Matrigel, s.c.) xenograft BALB/c nude mice (female, 18-20 g, 4-6 weeks of age) model[1]
Dosage: 10 mg/kg, 50 mg/kg
Administration: i.t. and PTI, once daily, 3 weeks
Result: Inhibited tumor growth, upregulated LATS1 and activated the Hippo pathway, was low in toxicity and did not change organ tissues.
分子量

406.39

Formula

C20H18N6O4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

DDO-4033 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DDO-4033DDO4033DDO 4033E1/E2/E3 EnzymeYAPE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseYes-associated proteinInhibitorRenal cell carcinomaA498 cellsBALB/c nude miceinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
DDO-4033
目录号：
HY-175247
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z