2. MAPK/ERK Pathway Stem Cell/Wnt
  3. ERK
  4. Endothelin-1 (1-31) (Human) TFA

Endothelin-1 (1-31) (Human) TFA 

目录号: HY-P4159A 纯度: 99.79%
COA 产品使用指南 技术支持

Endothelin-1 (1-31) (Human) TFA 是一种有效的血管收缩剂和血压升高剂。Endothelin-1 (1-31) (Human) TFA 来源于 chymase 对大 ET-1 的选择性水解。

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Custom Peptide Synthesis

Endothelin-1 (1-31) (Human) TFA

Endothelin-1 (1-31) (Human) TFA Chemical Structure

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规格 价格 是否有货 数量
1 mg ￥2300
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Customer Review

Other Forms of Endothelin-1 (1-31) (Human) TFA:

Endothelin-1 (1-31) (Human) TFA 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

查看 ERK 亚型特异性产品:

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ERK ERK1 ERK2 ERK5 ERK8

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Endothelin-1 (1-31) (Human) TFA is a potent vasoconstrictor and hypertensive agent. Endothelin-1 (1-31) (Human) TFA is derived from the selective hydrolysis of big ET-1 by chymase[1].

体外研究
(In Vitro)

Endothelin-1 (1-31) (Human) (100 pM-100 nM; 24 h) TFA 诱导人系膜细胞增殖[2]。
Endothelin-1 (1-31) (Human) (100 nM; 0-10 min) TFA 在人系膜细胞中诱导 ERK 激活[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Endothelin-1 (1-31) (Human) TFA 相关抗体:

Cell Proliferation Assay[2]

Cell Line: Human mesangial cells
Concentration: 100 pM-100 nM
Incubation Time: 24 h
Result: Caused an increase in [3H]-thymidine incorporation into the cells in a concentration-dependent manner.

Western Blot Analysis[2]

Cell Line: Human mesangial cells
Concentration: 100 nM
Incubation Time: 0, 5, 10, 15 and 30 min
Result: ERK activities rapidly increased 2.45-fold at 5 min and peaked at 10 min. The activities of both ERKs rapidly declined, returning to the baseline control value 30 min after stimulation.
体内研究
(In Vivo)

ET-1 (1-31) (100 nM; single dose) TFA 诱导小鼠肠系膜动脉收缩。收缩可能是由 ETA 受体介导的，并可能随着年龄的增长而增加。在目前的慢性糖尿病的情况下，男性和女性之间存在明显差异[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: ICR mice, Streptozocin (HY-13753)-induced diabetic model[1]
Dosage: 100 nM
Administration: In the organ bath, single dose
Result: In the 1-week control (but not diabetic) group, induced contraction and the contractile response was significantly greater in female mice than in male mice, and there was no significant difference in either male or female mice between the age-matched controls and the diabetic mice. In the 8-weeks group, the contraction was or tended to be increased compared with the corresponding 1-week group in all mice. Although in male mice this contraction was not different between control and diabetic groups, it was significantly greater in diabetic female mice than in the control female mice and in female diabetic mice than in male diabetic mice. The contraction was inhibited by ETA receptor inhibitor.
分子量

3742.18

Formula

C164H237F3N38O49S5
性状

固体

颜色

White to off-white

Sequence

Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp-Val-Asn-Thr-Pro-Glu-His-Val-Val-Pro-Tyr (Disulfide bridge:Cys1-Cys15;Cys3-Cys11)
Sequence Shortening

CSCSSLMDKECVYFCHLDIIWVNTPEHVVPY (Disulfide bridge:Cys1-Cys15;Cys3-Cys11)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

溶解性数据
细胞实验： 

H2O 中的溶解度 : 50 mg/mL (13.36 mM; adjust pH to 10 with NH4OH)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.2672 mL 1.3361 mL 2.6722 mL
5 mM 0.0534 mL 0.2672 mL 0.5344 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: 99.79%

COA (286 KB) LCMS (95 KB)

产品使用指南 (1538 KB)
参考文献

Endothelin-1 (1-31) (Human) TFA 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.2672 mL 1.3361 mL 2.6722 mL 6.6806 mL
5 mM 0.0534 mL 0.2672 mL 0.5344 mL 1.3361 mL
10 mM 0.0267 mL 0.1336 mL 0.2672 mL 0.6681 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Endothelin-1 (1-31) (Human)ERKExtracellular signal regulated kinasesvasoconstrictorhypertensivemesangial cellInhibitorinhibitorinhibit

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