2. Academic Validation
  3. Redox-based reagents for chemoselective methionine bioconjugation

Redox-based reagents for chemoselective methionine bioconjugation

  • Science. 2017 Feb 10;355(6325):597-602. doi: 10.1126/science.aal3316.
Shixian Lin 1,  Xiaoyu Yang 1 2,  Shang Jia 1,  Amy M Weeks 3,  Michael Hornsby 3,  Peter S Lee 3,  Rita V Nichiporuk 4,  Anthony T Iavarone 4,  James A Wells 3 5,  F Dean Toste 6 7,  Christopher J Chang 6 8 9 7
Affiliations

Affiliations

  • 1 Department of Chemistry, University of California, Berkeley, CA, USA.
  • 2 School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.
  • 3 Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, USA.
  • 4 California Institute for Quantitative Biosciences, University of California, Berkeley, CA, USA.
  • 5 Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA, USA.
  • 6 Department of Chemistry, University of California, Berkeley, CA, USA. chrischang@berkeley.edu fdtoste@berkeley.edu.
  • 7 Chemical Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • 8 Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
  • 9 Howard Hughes Medical Institute, University of California, Berkeley, CA, USA.
PMID: 28183972 DOI: 10.1126/science.aal3316
Abstract

Cysteine can be specifically functionalized by a myriad of acid-base conjugation strategies for applications ranging from probing protein function to antibody-drug conjugates and proteomics. In contrast, selective ligation to the Other sulfur-containing amino acid, methionine, has been precluded by its intrinsically weaker nucleophilicity. Here, we report a strategy for chemoselective methionine bioconjugation through redox reactivity, using oxaziridine-based reagents to achieve highly selective, rapid, and robust methionine labeling under a range of biocompatible reaction conditions. We highlight the broad utility of this conjugation method to enable precise addition of payloads to proteins, synthesis of antibody-drug conjugates, and identification of hyperreactive methionine residues in whole proteomes.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-D2910
    修饰剂
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z