2. Academic Validation
  3. Production of glutaric acid from 5-aminovaleric acid by robust whole-cell immobilized with polyvinyl alcohol and polyethylene glycol

Production of glutaric acid from 5-aminovaleric acid by robust whole-cell immobilized with polyvinyl alcohol and polyethylene glycol

  • Enzyme Microb Technol. 2019 Sep;128:72-78. doi: 10.1016/j.enzmictec.2019.05.003.
Soo-Yeon Yang 1,  Tae-Rim Choi 1,  Hye-Rim Jung 1,  Ye-Lim Park 1,  Yeong-Hoon Han 1,  Hun-Suk Song 1,  Shashi Kant Bhatia 2,  Kyungmoon Park 3,  Jung-Oh Ahn 4,  Woo-Young Jeon 4,  Jae-Seok Kim 5,  Yung-Hun Yang 6
Affiliations

Affiliations

  • 1 Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea.
  • 2 Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea; Institute for Ubiquitous Information Technology and Applications, Konkuk University, Seoul 05029, Republic of Korea.
  • 3 Department of Biological and Chemical Engineering, Hongik University, Sejong Ro 2639, Jochiwon, Sejong City, Republic of Korea.
  • 4 Biotechnology Process Engineering Center, Korea Research Institute Bioscience Biotechnology (KRIBB), Gwahangno, Yuseong-Gu, Daejeon 305-806, Republic of Korea.
  • 5 Department of Laboratory Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Republic of Korea.
  • 6 Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea; Institute for Ubiquitous Information Technology and Applications, Konkuk University, Seoul 05029, Republic of Korea. Electronic address: seokor@konkuk.ac.kr.
PMID: 31186113 DOI: 10.1016/j.enzmictec.2019.05.003
Abstract

Glutaric acid is an attractive C5 dicarboxylic acid with wide applications in the biochemical industry. Glutaric acid can be produced by fermentation and bioconversion, and several of its biosynthesis pathways have been well characterized, especially the simple pathway involving glutaric acid from l-lysine using 5-aminovaleric acid. We previously reported the production of glutaric acid using 5-aminovaleric acid and α-ketoglutaric acid by a whole-cell reaction, resulting in a high conversion yield. In this study, we sought to enhance the stability and reusability of this whole-cell system for realizing the efficient production of glutaric acid under harsh reaction conditions. To this end, various matrices were screened to immobilize Escherichia coli whole-cell overexpressing 4-aminobutyrate aminotransferase (GabT), succinate semi-aldehyde dehydrogenase (GabD), and NAD(P)H oxidase (NOX). We ultimately selected a PVA-PEG gel (LentiKats®) for cell entrapment, and several factors of the reaction were optimized. The optimal temperature and pH were 35 °C and 8.5, respectively. Treatment with Tween 80 as a surfactant, as well as additional NOX, was found to be effective. Under the optimized conditions, an immobilized cell retained 55% of its initial activity even after the eighth cycle, achieving 995.2 mM accumulated glutaric acid, whereas free cell lost most of their activity after only two cycles. This optimized whole-cell system can be used in the large-scale production of glutaric acid.

Keywords

Glutaric acid; Immobilization; LentiKats(®); Optimization.

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