1. Academic Validation
  2. Channel catfish virus entry into host cells via clathrin-mediated endocytosis

Channel catfish virus entry into host cells via clathrin-mediated endocytosis

  • Virus Res. 2022 Jul 2;315:198794. doi: 10.1016/j.virusres.2022.198794.
Hongxun Chen 1 Fei Yu 1 Jiehua Xu 1 Shuxin Li 1 Xiaodong Zhang 1 Lihui Meng 2 Kai Hao 1 Zhe Zhao 3
Affiliations

Affiliations

  • 1 Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, PR China.
  • 2 Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, PR China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China.
  • 3 Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, PR China. Electronic address: zhezhao@hhu.edu.cn.
Abstract

Channel catfish virus (CCV), an important member of the family Alloherpesviridae, causes a lethal Infection in channel catfish. As with most animal viruses, the initial step of Infection by CCV is entry into host cells, which is also a promising Antiviral target for CCV disease. This study investigated the mechanism of host cell invasion by CCV using a series of biochemical inhibitor assays in channel catfish cells. CCV Infection in host cells was does-dependently inhibited when cells were treated with endosomal acidification inhibitors (5 μM chloroquine, 50 nM bafilomycin A1, and 1 mM ammonium chloride) and hypertonic medium (50 mM sucrose) , which suggests that CCV invades host cells in a manner dependent on low-pH and the endocytic pathway. Moreover, when the cells were pretreated with inhibitors of clathrin-mediated endocytosis, including chlorpromazine (2 μM) and dynasore (50 μM), the CCV Infection in the host cells was strongly inhibited. In contrast, the destruction of cellular Cholesterol by methyl-β-cyclodextrin and nystatin and inhibition of macropinocytosis had no effect on viral entry. Altogether, these findings indicate that CCV infects host cells via clathrin-mediated endocytosis in a low-pH-dependent manner, suggesting that this CCV entry pathway offers an Antiviral target against CCV disease.

Keywords

Antiviral target; Channel catfish virus; Endocytosis; Virus entry.

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