1. Autophagy Anti-infection Immunology/Inflammation
  2. Parasite Autophagy SARS-CoV Toll-like Receptor (TLR) HIV Antibiotic
  3. Chloroquine

Chloroquine  (Synonyms: 氯喹)

目录号: HY-17589A 纯度: 99.82%
COA 产品使用指南

Chloroquine 是一种广泛用于疟疾和类风湿性关节炎的抗炎试剂。Chloroquine 是自噬 (autophagy) 和 Toll 样受体 (TLRs) 的抑制剂。Chloroquine 有效抑制 SARS-CoV-2 (COVID-19) 感染 (EC50=1.13 μM)。

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Chloroquine Chemical Structure

Chloroquine Chemical Structure

CAS No. : 54-05-7

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10 mM * 1 mL in DMSO ¥550
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50 mg ¥350
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100 mg ¥500
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200 mg ¥875
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500 mg ¥1750
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Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 516 篇科研文献

WB
Proliferation Assay
IF

    Chloroquine purchased from MCE. Usage Cited in: Immun Inflamm Dis. 2023 Apr 12; 11:e830.

    Chloroquine (10 μM; 30 min) reverses the effect of OmpA on autophagy by inhibiting the expression of LC3 in BMDCs.

    Chloroquine purchased from MCE. Usage Cited in: Immun Inflamm Dis. 2023 Apr 12; 11:e830.

    Chloroquine (10 μM; 30 min) reverses the effect of OmpA on autophagy by inhibiting the expression of Beclin1 and LC3II/I while increasing the expression of P62 in BMDCs.

    Chloroquine purchased from MCE. Usage Cited in: Prostate. 2019 Jan;79(1):44-53.  [Abstract]

    Treatment with sub-dose of Everolimus (10 nM), together with CQ (5 µM) increases the levels of Bax and cleaved PARP, and decreases the levels of Bcl-2 expression in both PC3 and LNPER cells.

    Chloroquine purchased from MCE. Usage Cited in: Oncogene. 2018 May;37(22):2936-2952.  [Abstract]

    IPZ-treated U87 and U251 cells are treated with or without Baf-A1 (5 nM), CQ (40 μM), or MG132 (1 μM) for 48 h, followed by western blots.

    Chloroquine purchased from MCE. Usage Cited in: Biomed Pharmacother. 2018 Dec;109:1617-1627.  [Abstract]

    GFP-LC3B-expressing LX-2 cells are treated with DMSO, 3-MA (10 mM), or Chloroquine (30 μM) alone or in the presence of Carvedilol (CVD, 10 μM) for 24 h. The conversion of LC3B-I to LC3B-II is evaluated using immunoblotting.

    Chloroquine purchased from MCE. Usage Cited in: Int J Mol Med. 2018 May;41(5):2535-2544.  [Abstract]

    MC3T3-E1 cells are treated with AICAR (10 μM) in the presence or absence of 3-MA (5 mM) or CQ (10 μM) for 24 h, after which western blot analysis is conducted.

    Chloroquine purchased from MCE. Usage Cited in: Sci Rep. 2018 Mar 7;8(1):4108.  [Abstract]

    L02 cells exposed to PA (200 μM) with different concentrations of Rapamycin (Rapa) or Chloroquine (CQ) for 24 h. Palmitate (PA) induced higher protein expression of LC3 II/I and p62 compared with control as indicated by western blot.

    Chloroquine purchased from MCE. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2018 Feb 1;50(2):144-155.  [Abstract]

    Raw264.7 macrophages treated without or with Rapamycin (1 μΜ) or Chloroquine (20 μΜ) for 48 h. Western blot shows the protein expression levels of Atg5, Beclin1, LC3, and p62/SQSMT1.

    Chloroquine purchased from MCE. Usage Cited in: J Pharmacol Exp Ther. 2018 Oct;367(1):20-27.  [Abstract]

    Protein expression of LC3B and p62 in Panc-1 cells treated with Carboxamidotriazole (CAI) and/or Chloroquine (CQ).

    Chloroquine purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2018 Sep 5;503(2):501-507.  [Abstract]

    Western blot analysis of autophagy markers and LAMP2 in mice with AAV-vaspin and Chloroquine (CQ) pretreatment after I/R (1d).

    Chloroquine purchased from MCE. Usage Cited in: Neurosci Lett. 2018 Aug 24;682:112-117.  [Abstract]

    After treatment of CDDP with or without the autophagy inhibitor CQ, the expression of LC3-II in the CQ+CDDP group is less than that in the CQ group but is higher than that in the CDDP group at 24 h and 96 h.

    Chloroquine purchased from MCE. Usage Cited in: Cell Physiol Biochem. 2018;48(6):2318-2336.  [Abstract]

    Cells are treated with Rapamycin (Rp; 10 μM)+Eicosapentaenoic acid (EPA; 40 μM) with or without Chloroquine (CQ; 5 μM) for 48 h. Cell extracts are prepared and subjected to western blot analysis.

    Chloroquine purchased from MCE. Usage Cited in: EBioMedicine. 2017 May;19:49-59.  [Abstract]

    mCherry-GFPLC3 puncta analysis in SPC-A-1 cells. Cells are transfected with mCherry-GFP-LC3 plasmid and incubated for 6 h. Next, cells are pretreated with or without Chloroquine (50 μM) for 1 h followed by stimulation of 5 μM Flu for 24 h or 36 h. Scale bar, 10 μm and 2 μm (magnified graph). The lysosomal inhibitor Chloroquine (CQ) raises the lysosomal pH and increases the autophagic flux.

    Chloroquine purchased from MCE. Usage Cited in: Sci Rep. 2017 Jun 7;7(1):2929.  [Abstract]

    p53 and Cell apoptosis. MCF7 and MDA-MB-231 cells are treated with 80 μM ω-3 FFAs, 20 μM ATRA alone or in combination for 48 h. The expression of PARP and p53 protein. β-Actin is used as an internal control.

    Chloroquine purchased from MCE. Usage Cited in: Oncotarget. 2017 Nov 22;8(65):109135-109150.  [Abstract]

    Cells are treated with RA (20 μM) plus ω-3 PUFAs (80 μM) with or without CQ (5 μM) for 24 h. Cell extracts are prepared and subjected to western blotting analysis.

    Chloroquine purchased from MCE. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2016 Dec;48(12):1075-1084.  [Abstract]

    The change of LC3 is analyzed by western blotting after 6 h hypoxia in the presence or absence of Chloroquine of WDR26-overexpressing H9c2 cells.

    Chloroquine purchased from MCE. Usage Cited in: Tumour Biol. 2016 Jul;37(7):8811-24.  [Abstract]

    Assays for autophagy activity. Immunoblot analysis for LC3B. Compared with the Lenti-NC cells, the Lenti-CD44v6 cells display a higher intensity of autophagy flux but not steady-state levels when treated with 5-FU (*P<0.001).
    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Chloroquine is an antimalarial and anti-inflammatory agent widely used to treat malaria and rheumatoid arthritis. Chloroquine is an autophagy and toll-like receptors (TLRs) inhibitor. Chloroquine is highly effective in the control of SARS-CoV-2 (COVID-19) infection in vitro (EC50=1.13 μM)[1][2][3][4].

    IC50 & Target[1][2][3][5]

    Plasmodium

     

    Malaria

     

    TLRs

     

    SARS-COV-2

     

    HIV-1

     

    体外研究
    (In Vitro)

    Chloroquine (CHQ, 20 μM) 抑制 IL-12p70 释放并降低活化的人单核细胞衍生的朗格汉斯样细胞 (MoLC) 的 Th1 启动能力。
    Chloroquine (20 μM) 增强 MoLC 中 IL-1 诱导的 IL-23 分泌,随后增加 CD4+ T 细胞释放 IL-17A[1]
    Chloroquine (25 μM) 在亲本 MDA-MB-231 细胞中抑制常氧和缺氧条件下的 MMP-9 mRNA 表达。Chloroquine 对 MMP-2、MMP-9 和 MMP-13 mRNA 表达具有细胞、剂量和缺氧依赖性作用[2]
    使用 IRS-954 或 Chloroquine 抑制 TLR7TLR9 在体外显著降低 HuH7 细胞增殖[3]
    Chloroquine (0.01-100 μM;48 小时) 有效阻断病毒感染 vero E6 细胞,抑制 SARS-CoV-2 感染具有低微摩尔浓度 (EC50=1.13 μM)。
    Chloroquine 通过增加病毒/细胞融合所需的内体 pH 值以及干扰 SARS-CoV 细胞受体的糖基化来阻断病毒感染[4]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内研究
    (In Vivo)

    Chloroquine (80 mg/kg,ip) 不会阻止原位小鼠模型中具有高或低 TLR9 表达水平的三阴性 MDA-MB-231 细胞的生长[2]
    使用 IRS-954 或 Chloroquine 抑制 TLR7TLR9 可显著抑制小鼠异种移植模型中的肿瘤生长。Chloroquine也显著抑制了 DEN/NMOR 大鼠模型中 HCC 的发展[3]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Clinical Trial
    分子量

    319.87

    Formula

    C18H26ClN3

    CAS 号
    性状

    固体

    颜色

    White to light yellow

    中文名称

    氯喹

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶解性数据
    In Vitro: 

    Ethanol 中的溶解度 : 100 mg/mL (312.63 mM; 超声助溶)

    DMSO 中的溶解度 : 50 mg/mL (156.31 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 3.1263 mL 15.6314 mL 31.2627 mL
    5 mM 0.6253 mL 3.1263 mL 6.2525 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
    ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (7.82 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

      生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (7.82 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。

      20% SBE-β-CD in Saline 的配制(4°C,储存一周):2 g SBE-β-CD(磺丁基醚 β-环糊精)粉末定容于 10 mL 的生理盐水中,完全溶解至澄清透明。

    以下溶解方案,请直接配置工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂: 50% PEG300    50% Saline

      Solubility: 10 mg/mL (31.26 mM); 悬浊液; Need ultrasonic and warming and heat to 44°C

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    请输入您的动物体内配方组成:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
    连续给药周期超过半月以上,请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.82%

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO / Ethanol 1 mM 3.1263 mL 15.6314 mL 31.2627 mL 78.1568 mL
    5 mM 0.6253 mL 3.1263 mL 6.2525 mL 15.6314 mL
    10 mM 0.3126 mL 1.5631 mL 3.1263 mL 7.8157 mL
    15 mM 0.2084 mL 1.0421 mL 2.0842 mL 5.2105 mL
    20 mM 0.1563 mL 0.7816 mL 1.5631 mL 3.9078 mL
    25 mM 0.1251 mL 0.6253 mL 1.2505 mL 3.1263 mL
    30 mM 0.1042 mL 0.5210 mL 1.0421 mL 2.6052 mL
    40 mM 0.0782 mL 0.3908 mL 0.7816 mL 1.9539 mL
    50 mM 0.0625 mL 0.3126 mL 0.6253 mL 1.5631 mL
    60 mM 0.0521 mL 0.2605 mL 0.5210 mL 1.3026 mL
    80 mM 0.0391 mL 0.1954 mL 0.3908 mL 0.9770 mL
    100 mM 0.0313 mL 0.1563 mL 0.3126 mL 0.7816 mL
    Help & FAQs
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    产品名称:
    Chloroquine
    目录号:
    HY-17589A
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