2. Academic Validation
  3. Hydrophilic nanofibers with aligned topography modulate macrophage-mediated host responses via the NLRP3 inflammasome

Hydrophilic nanofibers with aligned topography modulate macrophage-mediated host responses via the NLRP3 inflammasome

  • J Nanobiotechnology. 2023 Aug 14;21(1):269. doi: 10.1186/s12951-023-02024-9.
Yiming Ren # 1 2,  Yi Chen # 3,  Wei Chen # 1 2,  Haotian Deng 1 2,  Peiqi Li 1 2,  Yubo Liu 1,  Cangjian Gao 2,  Guangzhao Tian 1 2,  Chao Ning 2,  Zhiguo Yuan 2,  Xiang Sui 2,  Shuyun Liu 4 5,  Quanyi Guo 6 7
Affiliations

Affiliations

  • 1 School of Medicine, Nankai University, Tianjin, 300071, China.
  • 2 Institute of Orthopedics, First Medical Center, Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma and War Injuries PLA, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, Beijing, 100853, China.
  • 3 Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 33 Badachu Road, Shijingshan District, Beijing, 100144, China.
  • 4 School of Medicine, Nankai University, Tianjin, 300071, China. clear_ann@163.com.
  • 5 Institute of Orthopedics, First Medical Center, Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma and War Injuries PLA, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, Beijing, 100853, China. clear_ann@163.com.
  • 6 School of Medicine, Nankai University, Tianjin, 300071, China. doctorguo_301@163.com.
  • 7 Institute of Orthopedics, First Medical Center, Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma and War Injuries PLA, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, Beijing, 100853, China. doctorguo_301@163.com.
  • # Contributed equally.
PMID: 37574546 DOI: 10.1186/s12951-023-02024-9
Abstract

Successful biomaterial implantation requires appropriate immune responses. Macrophages are key mediators involved in this process. Currently, exploitation of the intrinsic properties of biomaterials to modulate macrophages and immune responses is appealing. In this study, we prepared hydrophilic nanofibers with an aligned topography by incorporating polyethylene glycol and polycaprolactone using axial electrospinning. We investigated the effect of the nanofibers on macrophage behavior and the underlying mechanisms. With the increase of hydrophilicity of aligned nanofibers, the inflammatory gene expression of macrophages adhering to them was downregulated, and M2 polarization was induced. We further presented clear evidence that the inflammasome NOD-like receptor thermal protein domain associated protein 3 (NLRP3) was the cellular sensor by which macrophages sense the biomaterials, and it acted as a regulator of the macrophage-mediated response to foreign bodies and implant integration. In vivo, we showed that the fibers shaped the implant-related immune microenvironment and ameliorated peritendinous adhesions. In conclusion, our study demonstrated that hydrophilic aligned nanofibers exhibited better biocompatibility and immunological properties.

Keywords

Hydrophilicity; Immunomodulation; Macrophage; NLRP3 inflammasome; Peritendinous adhesion; Topography.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z