1. Membrane Transporter/Ion Channel Immunology/Inflammation Anti-infection Apoptosis Stem Cell/Wnt
  2. Sodium Channel NOD-like Receptor (NLR) Bacterial Apoptosis Antibiotic Pyroptosis Wnt β-catenin
  3. Nigericin

Nigericin  (Synonyms: 尼日利亚霉素)

目录号: HY-127019
产品使用指南

Nigericin 是一种抗生素 (antibiotic),源自 Streptomyces hygroscopicus,充当 K+/H+ 离子载体 (K+/H+ ionophore),促进 K+/H+ 跨线粒体膜交换。Nigericin 通过降低细胞内 pH (pHi) 和 Wnt/β-catenin 通路信号而显示出良好的抗癌活性。Nigericin 在 TNBC 中通过 caspase 1/GSDMD 途径诱导细胞焦亡 (pyroptosis)[3]

该游离形式化合物不稳定,推荐具有相同生物学活性的稳定盐形式 Nigericin sodium salt

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

Nigericin Chemical Structure

Nigericin Chemical Structure

CAS No. : 28380-24-7

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Nigericin 的其他形式现货产品:

Customer Review

Other Forms of Nigericin:

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 97 篇科研文献

WB

    Nigericin purchased from MCE. Usage Cited in: Phytomedicine. 2023 Mar 5;113:154743.  [Abstract]

    Priming with LPS (1 μg/mL; 4 h) and activating with Nigericin (10 μM; 2 h) induces NLRP3 expression, caspase-1 cleavage, and GSDMD cleavage in BMDMs.

    Nigericin purchased from MCE. Usage Cited in: Environ Toxicol. 2023 Mar 29.  [Abstract]

    Nigericin (1.38mM; 2 μg/2 μL; 10 min) signifcantly increases the expression of IL-1β, IL-18, and GSDMD N-terminal in rats

    Nigericin purchased from MCE. Usage Cited in: J Cell Mol Med. 2020 Jul;24(14):8078-8090.  [Abstract]

    LPS + Nigericin is used to induce pyroptosis as a positive control group.
    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Nigericin is an antibiotic derived from Streptomyces hygroscopicus that act as a K+/H+ ionophore, promoting K+/H+ exchange across mitochondrial membranes. Nigericin shows promising anti-cancer activities through decreasing intracellular pH (pHi), and inactivation of Wnt/β-catenin signals. Nigericin induces pyroptosis through caspase 1/GSDMD in TNBC[1][2][3][4][5][6][7].

    IC50 & Target

    NLRP3

     

    体外研究
    (In Vitro)

    Nigericin (0-4 μg/ml;24 小时) 通过 caspase 1/GSDMD 通路在 TNBC 细胞中诱导细胞焦亡 (pyroptosis)[6]
    Nigericin (0-0.25 μg/ml;24 小时) 是一种针对革兰氏阳性菌的抗生素[4]
    Nigericin (0-0.25 μg/ml;24 小时) 抑制 H460 细胞中的 Wnt/β-catenin 通路,发挥抗癌作用[7].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[4]

    Cell Line: S. aureus, Staphylococcus epidermidis, Enterococcus faecalis, E. faecium, S. pneumoniae) , and Streptococcus agalactiae
    Concentration: 0 μg/ml, 0.05 μg/ml, 0.125 μg/ml, 0.25 μg/ml
    Incubation Time: 24 h
    Result: Exhibited potent activity against these clinical MDR strains, with MIC values ranging from 0.004-0.25 mg/ml.

    Western Blot Analysis[6]

    Cell Line: MDA-MB-231, and 4T1 cells
    Concentration: 0 μg/ml, 2 μg/ml, 4 μg/ml
    Incubation Time: 24 h
    Result: Showed increasing in the proteins level of caspase 1 and N-GSDMD.

    Cell Viability Assay[7]

    Cell Line: H460 cells
    Concentration: 0.5 μM, 1 μM, 2.5 μM
    Incubation Time: 24 h
    Result: Showed downregulating in the expression of proteins of the canonical Wnt (LRP6, Wnt5a/b, and β-catenin) signaling pathway.
    体内研究
    (In Vivo)

    Nigericin (1 mg/kg;腹膜注射;每 12 h 一次,持续 3 天) 可减少小鼠 S. aureus USA300 菌的感染[4]
    Nigericin (0.025 mg/kg;皮下注射;每两天一次共四周) 协同 anti-PD-1 显示出协同抗癌作用[6]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: BALB/c Mice injected orthotopically with 4T1 cells[6]
    Dosage: 0.025 mg/kg
    Administration: Subcutaneous injection (s.c.)
    Result: Showed combination with anti-PD-1 antibody almost completely suppressed tumor growth.
    Animal Model: Mice Infected with S. aureus USA300[4]
    Dosage: 1 mg/kg
    Administration: Intraperitoneal injection (i.p.)
    Result: Showed reduction in the bacterial burden to 1,000-10,000-fold in the major organs.
    分子量

    724.96

    Formula

    C40H68O11

    CAS 号
    中文名称

    尼日利亚霉素

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    Please store the product under the recommended conditions in the Certificate of Analysis.

    纯度 & 产品资料
    参考文献
    • 摩尔计算器

    • 稀释计算器

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量   浓度   体积   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    您最近查看的产品:

    Your information is safe with us. * Required Fields.

       产品名称:

     

    * 需求量:

    * 客户姓名:

     

    * Email:

    * 电话:

     

    * 公司或机构名称:

       留言给我们:

    Bulk Inquiry

    Inquiry Information

    产品名称:
    Nigericin
    目录号:
    HY-127019
    需求量: