2. Academic Validation
  3. Purification of creatine kinase-BB isoenzymes from human and canine tissues

Purification of creatine kinase-BB isoenzymes from human and canine tissues

  • Clin Chim Acta. 1984 Sep 15;142(1):61-71. doi: 10.1016/0009-8981(84)90101-3.
J Miller,  R Wei
PMID: 6478624 DOI: 10.1016/0009-8981(84)90101-3
Abstract

We describe a procedure for the purification of Creatine Kinase BB isoenzyme from canine and human brain tissue. The purification involves sequentially 50-70% (NH4)2SO4 fractionation, DEAE-Sephacel, Blue-Sepharose CL-6B, and chromatofocusing chromatography. This method produces a homogeneous Protein Purification as verified by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing chromatography.

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