RALA peptide 

RALA peptide is a cationic amphiphilic delivery agent targeting cell membranes, which forms nanoparticles through electrostatic interactions with anionic drugs. RALA peptide has pH-responsive binding properties, which can enhance the α-helical conformation in an acidic environment and destroy the endosomal membrane, promote the release of drugs into the cytoplasm, and exert efficient intracellular delivery activity. RALA peptide can be used in cancer research (enhancing the activity of bisphosphonates against prostate cancer and breast cancer cells) and bone tissue engineering (promoting osteoblast collagen deposition and extracellular matrix mineralization)^[1][2][3].

RALA peptide (与双膦酸盐质量比 10:1；体外 6 h 处理后培养 72 h) 与双膦酸盐形成的纳米颗粒可显著增强对 PC3 前列腺癌细胞和 MDA-MB-231 乳腺癌细胞的抗增殖活性^[1]。
RALA peptide (与 α-TCP 质量比 5:1；体外 24 h 转染后培养 28 d) 复合 α-TCP 形成的纳米颗粒可促进 MG-63 成骨细胞的 I 型胶原表达和沉积，增强猪间充质干细胞的细胞外基质矿化^[2][3]。
RALA peptide (与 α-TCP 质量比 5:1；体外培养 2-4 周) 递送 α-TCP 至间充质干细胞后，显著上调 BMP-2 和核心成骨基因表达，加速工程化水凝胶内的矿化进程^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

RALA peptide (10 μg 阿仑膦酸盐/次；瘤内注射；3 次/周；共 3 周) 在 PC3 荷瘤小鼠模型中显著延缓肿瘤生长，延长小鼠中位生存时间至 26 天 (对照组 16 天)，证实 RALA/阿仑膦酸盐纳米颗粒通过增强药物递送提升体内抗肿瘤效果^[1]。
RALA peptide (复合 α-TCP 纳米颗粒；皮下植入；单次植入；体内植入 8 周) 在 Balb/C 裸鼠模型中促进成骨分化和血管化实现高效骨组织再生，诱导间充质干细胞负载的水凝胶内骨组织形成，矿化体积与生长因子递送组无显著差异，表明 RALA 介导的 α-TCP 递送可替代生长因子促进体内骨再生^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

3327.92

C₁₄₄H₂₄₈N₅₄O₃₅S

1613231-49-4

固体

White to off-white

Trp-Glu-Ala-Arg-Leu-Ala-Arg-Ala-Leu-Ala-Arg-Ala-Leu-Ala-Arg-His-Leu-Ala-Arg-Ala-Leu-Ala-Arg-Ala-Leu-Arg-Ala-Cys-Glu-Ala

WEARLARALARALARHLARALARALRACEA

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

• [1]. Massey AS, et al. Potentiating the Anticancer Properties of Bisphosphonates by Nanocomplexation with the Cationic Amphipathic Peptide, RALA. Mol Pharm. 2016 Apr 4;13(4):1217-28.  [Content Brief]

  [2]. O'Doherty M, et al. Improving the Intercellular Uptake and Osteogenic Potency of Calcium Phosphate via Nanocomplexation with the RALA Peptide. Nanomaterials (Basel). 2020 Dec 7;10(12):2442  [Content Brief]

  [3]. Sathy BN, et al. RALA complexed α-TCP nanoparticle delivery to mesenchymal stem cells induces bone formation in tissue engineered constructs in vitro and in vivo. J Mater Chem B. 2017 Mar 7;5(9):1753-1764.  [Content Brief]