FLT3LG 蛋白, Human (sf9, His)

FLT3LG Proteinas, a potent stimulator of early hematopoietic cell proliferation, activates FLT3 and synergizes with colony-stimulating factors and interleukins. As a homodimer, especially in isoform 2, it crucially promotes expansion and differentiation of hematopoietic progenitor cells. FLT3LG's collaborative signaling with other molecules underscores its significance in regulating hematopoiesis and maintaining hematopoietic system balance. FLT3LG Protein, Human (sf9, His) is the recombinant human-derived FLT3LG protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of FLT3LG Protein, Human (sf9, His) is 159 a.a., with molecular weight of ~27 kDa.

FLT3LG 蛋白通过激活 FLT3 作为早期造血细胞增殖的有效刺激剂，与各种集落刺激因子和白细胞介素结合时显示出协同效应。这种同型二聚体蛋白，尤其是亚型 2，在促进造血祖细胞的扩增和分化中发挥着至关重要的作用。它激活FLT3并与其他信号分子协作的能力强调了其在调节造血和维持造血系统平衡方面的重要性。

Measured in a cell proliferation assay using BaF3 mouse pro­B cells transfected with mouse Flt­3 and the ED₅₀ is typically 2-11 ng/mL.

Human

Sf9 insect cells

N-His

P49771-1 (T27-P185)

2323  [NCBI]

TQDCSFQHSPISSDFAVKIRELSDYLLQDYPVTVASNLQDEELCGGLWRLVLAQRWMERLKTVAGSKMQGLLERVNTEIHFVTKCAFQPPPSCLRFVQTNISRLLQETSEQLVALKPWITRQNFSRCLELQCQPDSSTLPPPWSPRPLEATAPTAPQPP

Approximately 27 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 300 mM NaCl, 10% Glycerol, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.