1. 重组蛋白
  2. Cytokines and Growth Factors
  3. FLT3LG 蛋白, Mouse (HEK293, Fc)

FLT3LG 蛋白, Mouse (HEK293, Fc)

目录号: HY-P73067
COA 产品使用指南

FLT3LG Proteinas 是一种有效的刺激剂,可激活 FLT3,与集落刺激因子和白细胞介素协同作用。其同型二聚体形式,尤其是可溶性亚型,有效促进造血祖细胞的扩增和分化。该蛋白质的重要性在于协调造血系统内的关键过程。FLT3LG 蛋白, Mouse (HEK293, Fc) 是重组的 FLT3LG 蛋白,由 HEK293 表达,带有 C-hFc 标签。FLT3LG 蛋白, Mouse (HEK293, Fc) 全长 162 个氨基酸,分子量分别约为 ~35 & 55-65 kDa。

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规格 价格 是否有货 数量
10 μg ¥2050 In-stock
20 μg ¥3480 In-stock
100 μg ¥9750 In-stock
> 100 μg   询价  

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描述

FLT3LG Proteinas, a potent stimulator, activates FLT3, synergizing with colony-stimulating factors and interleukins. Its homodimeric form, especially in the soluble isoform, effectively promotes the expansion and differentiation of hematopoietic progenitor cells. The protein's significance lies in orchestrating key processes within the hematopoietic system. FLT3LG Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived FLT3LG protein, expressed by HEK293 , with C-hFc labeled tag. The total length of FLT3LG Protein, Mouse (HEK293, Fc) is 162 a.a., with molecular weight of ~35 & 55-65 kDa, respectively.

研究背景

FLT3LG 蛋白充当有效的刺激剂,通过激活 FLT3 促进早期造血细胞的增殖。这种同型二聚体蛋白表现出协同效应,特别是在其可溶性异构体中,可与各种集落刺激因子和白细胞介素有效地协同作用。它在促进造血祖细胞扩增和分化方面的作用强调了它在协调造血系统内关键过程中的重要性。

生物活性

1.Measured in a cell proliferation assay using BaF3 mouse pro­B cells transfected with mouse Flt­3 and the ED50 is typically 1-30 ng/mL.
2.Mouse FLT3 Ligand, hFc Tag captured on CM5 Chip via Protein A can bind Human FLT3, His Tag with an affinity constant of 13.81 nM as determined in SPR assay (Biacore T200).

种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

P49772-1 (G27-R188)

基因 ID

14256  [NCBI]

蛋白结构
N-term
FLT3LG (G27-R188)
Accession # P49772-1
hFc
C-term
同用名
Fms-related tyrosine kinase 3 ligand; Flt3 Ligand; Flt3L; SL Cytokine; FLT3LG
氨基酸序列

GTPDCYFSHSPISSNFKVKFRELTDHLLKDYPVTVAVNLQDEKHCKALWSLFLAQRWIEQLKTVAGSKMQTLLEDVNTEIHFVTSCTFQPLPECLRFVQTNISHLLKDTCTQLLALKPCIGKACQNFSRCLEVQCQPDSSTLLPPRSPIALEATELPEPRPR

分子量

Approximately 35&55-65 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

FLT3LG Protein, Mouse (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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