1. 重组蛋白
  2. Cytokines and Growth Factors
  3. FLT3LG 蛋白, Human (CHO)

FLT3LG 蛋白, Human (CHO)

目录号: HY-P7111
COA 产品使用指南

FLT3LG 蛋白, Human (CHO)是一种细胞因子,能够促进造血干细胞及其祖细胞的再生和分化。

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规格 价格 是否有货 数量
10 μg ¥1900 In-stock
50 μg ¥5400 In-stock
100 μg ¥8900 询价
> 100 μg   询价  

* Please select Quantity before adding items.

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  • 参考文献

描述

FLT3LG Protein, Human (CHO) is a cytokine, which can promote the generation and differentiation of hematopoietic stem cells and their progenitor cells.

研究背景

Fms相关酪氨酸激酶3配体是一种细胞因子,能够促进造血干细胞及其祖细胞的生成和分化。Fms相关酪氨酸激酶3配体的mRNA在某些组织中高表达,包括外周血单核细胞、心脏、胎盘、肺、脾和胸腺组织,在非造血组织中也有一定程度的表达,包括前列腺、睾丸、卵巢、肠道、肝脏、肾脏和骨骼肌[1]

生物活性

The ED50 is <1 ng/mL as measured by human AML5 cells, corresponding to a specific activity of >1 × 106 units/mg.

种属

Human

表达系统

CHO

标签

Tag Free

蛋白编号

P49771 (T27-P185)

基因 ID
蛋白结构
N-term
FLT3LG (T27-P185)
Accession # P49771
C-term
中文名
重组人 Fms 样酪氨酸激酶 3 配体 (CHO 细胞表达)
同用名
rHuFlt-3 Ligand; Flt3 ligand; FL; SL cytokine; Flt-3L
氨基酸序列

TQDCSFQHSPISSDFAVKIRELSDYLLQDYPVTVASNLQDEELCGGLWRLVLAQRWMERLKTVAGSKMQGLLERVNTEIHFVTKCAFQPPPSCLRFVQTNISRLLQETSEQLVALKPWITRQNFSRCLELQCQPDSSTLPPPWSPRPLEATAPTAPQPP

分子量

18-22 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

FLT3LG Protein, Human (CHO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FLT3LG Protein, Human (CHO)
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