1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-13
  5. IL-13 蛋白, Human (HEK293, His)

IL-13 蛋白, Human (HEK293, His)

目录号: HY-P70568
COA 产品使用指南

IL-13 蛋白, Human (HEK293, His)是一种细胞因子,影响单核细胞形态,生长和表面抗原表达和表型,并引发 B 细胞增殖。IL-13, Human (HEK293, His) 是一种在 HEK 293 细胞中表达的,末端有 His 标签的重组人白细胞介素-13 (rhIL-13)。

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2 μg ¥600 In-stock
10 μg ¥1700 In-stock
50 μg ¥5100 In-stock
100 μg   询价  

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  • 生物活性

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描述

IL-13 Protein, Human (HEK293, His) is a cytokine which affects the morphology, growth, and surface antigen expression and phenotype of monocytes and elicits B cell proliferation. IL-13 Protein, Human (HEK293, His) is a recombinant human interleukin-13 (rhIL-13) expressed in HEK 293 cells with a His tag.

研究背景

Interleukin-13 是一种细胞因子,由活化的 T 淋巴细胞分泌,主要影响单核细胞、巨噬细胞和 B 细胞。圆二色光谱证实 IL-13 属于细胞因子的 α 螺旋家族。Interleukin-13 影响单核细胞的形态、生长、表面抗原表达和表型,并引发 B 细胞增殖、Ig 类别转换为 IgE 合成以及 IgG4IgM 的产生增强。在人类巨噬细胞和单核细胞中,hIL-13 已被证明可以抑制 HIV 复制。人 IL-13 还可抑制 LPS 暴露诱导的促炎细胞因子,表明作为抗炎剂具有潜在的治疗应用[1]

生物活性

The cell proliferation assay using TF-1 human erythroleukemic cells has an ED50 value of 1.5-8.61 ng/mL.

  • Measured in a cell proliferation assay using TF-1 human erythroleukemic cell.The ED50 for this effect is 6.27 ng/mL, corresponding to a specific activity is 1.59×105 U/mg.
种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

AAH96139 (G35-N146)

基因 ID
蛋白结构
N-term
IL-13 (G35-N146)
Accession # AAH96139
6*His
C-term
同用名
Interleukin-13; IL-13
氨基酸序列

GPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCAALESLINVSGCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKKLFREGRFN

分子量

Approximately 17-30 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IL-13 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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