IL-13 蛋白, Human

IL-13 Protein is a cytokine which is secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosinophils and nuocytes. IL-13 affects the morphology, growth, and surface antigen expression and phenotype of monocytes and elicits B cell proliferation. IL-13 Protein, Human is the recombinant human-derived IL-13 protein, expressed by E. coli , with tag free. The total length of IL-13 Protein, Human is 112 a.a., with molecular weight of ~12.3 kDa.

白细胞介素-13 (IL-13) 是一种细胞因子，由 2 型辅助 T (Th2) 细胞、CD4 细胞、自然杀伤 T 细胞、肥大细胞、嗜碱性粒细胞、嗜酸性粒细胞和核细胞分泌。IL-13 是 IgE 合成、杯状细胞增生、粘液分泌过多、气道高反应性、纤维化和几丁质酶上调的核心调节因子。圆二色光谱证实 IL-13 属于细胞因子的 α 螺旋家族。IL-13 与 IL2 协同调节干扰素-γ 合成。IL-13 通过 IL4R 链和 IL13RA1 链发挥生物学作用，激活 JAK1、TYK2 和 STAT6。Interleukin-13 影响单核细胞的形态、生长、表面抗原表达和表型，刺激B细胞增殖，并激活嗜酸性粒细胞、嗜碱性粒细胞和肥大细胞。在人类巨噬细胞和单核细胞中，hIL-13 已被证明可以抑制 HIV 复制。Human IL-13 还可抑制 LPS 暴露诱导的促炎细胞因子，表明作为抗炎剂的潜在治疗应用^{[1][2][3][4][5][6]}。

The cell proliferation assay using human TF-1 cells has an ED₅₀ value of less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.

Human

E. coli

Tag Free

P35225 (G35-N146)

3596  [NCBI]

GPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCAALESLINVSGCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKKLFREGRFN

Approximately 12.3 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.2, with 5% trehalose.

<1 EU/μg; determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.