1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-13
  5. IL-13 蛋白, Human

IL-13 是一种多功能细胞因子。IL-13 通过结合细胞表面的 II 型 IL-4 受体复合物 (IL-4Rα/IL-13Rα1),激活 JAK-STAT6 信号通路,调控基因表达。IL-13 可诱导单核细胞 CD23 表达、B 细胞 IgE 合成、成纤维细胞 eotaxin 分泌及气道平滑肌细胞钙流增强。IL-13 可促进小鼠气道炎症。IL-13 蛋白, Human 是重组的 IL-13 蛋白,由 E. coli 表达,不带标签。

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5 μg ¥1200 In-stock
10 μg ¥1950 In-stock
50 μg ¥5560 In-stock
100 μg ¥9000 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

IL-13 is a multifunctional cytokine. IL-13 activates the JAK-STAT6 signaling pathway and regulates gene expression by binding to the type II IL-4 receptor complex (IL-4Rα/IL-13Rα1) on the cell surface. IL-13 can induce monocyte CD23 expression, B cell IgE synthesis, fibroblast eotaxin secretion, and enhanced calcium flux in airway smooth muscle cells. IL-13 promotes airway inflammation in mice. IL-13 Protein, Human is a recombinant IL-13 protein expressed by E. coli without a tag.

研究背景

IL-13 属于白细胞介素 (IL) 家族[1]IL-13 是一种多功能细胞因子[1][2][5]IL-13 通过结合细胞表面的 II 型 IL-4 受体复合物 (IL-4Rα/IL-13Rα1),激活 JAK-STAT6 信号通路,调控基因表达,其效应具有细胞类型特异性 (如在造血细胞中依赖 γc 链,在非造血细胞中依赖 IL-13Rα1)[1][5]IL-13 可诱导单核细胞 CD23 表达、B 细胞 IgE 合成、成纤维细胞 eotaxin 分泌及气道平滑肌细胞钙流增强。IL-13 可促进小鼠气道嗜酸性粒细胞浸润、AHR 和肝纤维化[1][3][5]IL-13 在多种组织和细胞中表达,包括肺、肝、食道等组织的免疫细胞 (如巨噬细胞、单核细胞、B 细胞) 和结构细胞 (如成纤维细胞、气道平滑肌细胞) [1][3][5]IL-13, Human 与小鼠 IL-13 在氨基酸序列上具有约 60% 的同源性,但功能存在种属差异 (如人 IL-13 不激活小鼠 IL-13 受体)[5]IL-13, Human 是由 132 个氨基酸组成的非糖基化蛋白,分子量约 10 kDa[4]

体外研究
(In Vitro)

IL-13 Protein, Human (0.8 nM) 诱导正常人肺成纤维细胞 (NHLF) 中 eotaxin-1 的产生[5]

体内研究
(In Vivo)

IL-13 Protein, Human (2 μg) 在小鼠空气囊模型中诱导炎症,引起白细胞和嗜酸性粒细胞浸润[5]

生物活性

The cell proliferation assay using human TF-1 cells has an ED50 value of less than 5 ng/mL.

  • Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1.195 ng/mL, corresponding to a specific activity is 8.37×105 U/mg.
种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P35225 (G35-N146)

基因 ID
蛋白结构
N-term
IL-13 (G35-N146)
Accession # P35225
C-term
蛋白长度

Partial

中文名
白介素-13 蛋白
同用名
Interleukin-13; IL-13
氨基酸序列

GPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCAALESLINVSGCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKKLFREGRFN

分子量

Approximately 12.3 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.2, with 5% or 8% trehalose.

内毒素含量

<1 EU/μg; determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IL-13 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-13 Protein, Human
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