Noggin 蛋白, Human (HEK293)

Noggin Protein, Human (HEK293) is a recombinant protein produced by HEK293 cells. Noggin is a potent bone morphogenetic protein (BMP) antagonist capable of inhibiting vasculogenesis even in the presence of provasculogenic VEGF and FGF-2^[1].

Noggin 是一种糖基化半胱氨酸结趋化因子蛋白，作为转化生长因子 (TGF)β 超家族成员的细胞外负调节因子。与其他几种拮抗剂一起，Noggin 阻断多能骨形态发生蛋白 (BMP) 信号，该信号局部作用于靶细胞，影响细胞存活、增殖和分化。Noggin 通过形成中和复合物来抑制 BMP，从而阻止 BMP 与 BMP 受体结合^[1]。

1.Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The IC₅₀ is < 1.97 ng/mL, corresponding to a specific activity of > 5.08×10⁵ units/ mg.
2.Measured by its ability to inhibit BMP-2-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells The ED₅₀ for this effect is <2 μg/mL in the presence of 2000 ng/mL of Recombinant Human BMP-2.
3.Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells The ED₅₀ for this effect is <2 ng/mL in the presence of 50 ng/mL of Recombinant Human BMP-4.
4.Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 3.0 ng/mL in the presence of 30 ng/mL of Recombinant Human BMP-4, corresponding to a specific activity is 3.33×10⁵ units/mg.

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  "Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The IC₅₀ is 1.523 ng/mL, corresponding to a specific activity of 6.6×10⁵ units/ mg.

Human

HEK293

Tag Free

Q13253 (Q28-C232)

9241  [NCBI]

QHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC

Approximately 28-32 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 500 mM NaCl, 2 mM EDTA, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Kang HW, et al. In vitro and In vivo imaging of antivasculogenesis induced by Noggin protein expression in human venous endothelial cells. FASEB J. 2009;23(12):4126-4134.  [Content Brief]