1. Apoptosis
    Autophagy
  2. c-Myc
    Autophagy
  3. Stauprimide

Stauprimide 

目录号: HY-N6747
产品使用指南

Stauprimide 是一种促进胚胎干细胞 (ESC) 分化的星形孢菌素类似物。Stauprimide 与 MYC 转录因子 NME2 结合并阻断其在 ESC 中的核定位,导致 MYC 转录的下调, 是一种非广谱抑制剂。

MCE 的所有产品仅用作科学研究,我们不为任何个人用途提供产品和服务

Stauprimide Chemical Structure

Stauprimide Chemical Structure

CAS No. : 154589-96-5

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

Size Stock
100 mg   Get quote  
250 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Top Publications Citing Use of Products
  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

Description

Stauprimide is a staurosporine analog that promotes embryonic stem cell (ESC) differentiation. Stauprimide is a non-broad spectrum inhibitor that binds to the MYC transcription factor NME2 and blocks its nuclear localization in ESCs, which results in down-regulation of MYC transcription[1].

In Vitro

Stauprimide (10 μM; 6 hours) suppresses MYC transcription in the majority of cell lines tested with an EC50 range of 30 nM-8 μM, and decreases MYC levels between 15% to over 90%[1].
Stauprimide (2-8 μM; 24-72 hours) down-regulates MYC leads to the inhibition of cell proliferation in vitro with an IC50 of 780 nM in RXF 393 cells[1].
Stauprimide (5 μM; 3 hours) suppresses MYC Transcription by decreasing NME2 Nuclear Translocation[1].
Stauprimide (4-10 μM; 6 hours) acts with different EC50s and with different degrees of maximal MYC mRNA down-regulation in different cell lines[1].

Cell Proliferation Assay[1]

Cell Line: Renal cancer cell line RXF 393 cells
Concentration: 2 μM, 4 μM, 8 μM
Incubation Time: 24 hours, 48 hours, 72 hours
Result: Inhibited cell proliferation at concentrations of 2-8 μM.

Western Blot Analysis[1]

Cell Line: Renal cancer cell line RXF 393 cells and CAKI-1 cells
Concentration: 5 μM
Incubation Time: 3 hours
Result: Decreased nuclear localized NME2.

RT-PCR[1]

Cell Line: CA46 cells; Ramos cells; RXF393 cells; TK10 cells; KG1A cells; CAKI-1 cells
Concentration: 4μM, 6μM, 8μM, 10μM
Incubation Time: 6 hours
Result: Suppressed MYC transcription in KG1A cells with an EC50 of 400±50 nM and a suppression of 90%; CA46 cells were resistant to stauprimide.
In Vivo

Stauprimide (oral administration; 50 mg/kg; once daily; 30 days, 55 days) blocks tumor growth, reduces MYC protein levels in xenograft mouse with RXF 393 or CAKI-1 cells and inhibits MYC transcription in the RXF 393 tumor[1].

Animal Model: Xenograft tumor models with RXF 393 and CAKI-1 cells in NOD/SCID mice[1]
Dosage: 50 mg/kg
Administration: Oral administration; 50 mg/kg; once daily; 30 days, 55 days
Result: Blocked tumor growth in mice injected with either RXF 393 or CAKI-1 cells during the dosing periods.
Molecular Weight

584.62

Formula

C₃₅H₂₈N₄O₅

CAS No.

154589-96-5

SMILES

C[C@@]([C@@H]1OC)(O[C@](N2C3=CC=CC=C43)([H])C[C@H]1N(C)C(C5=CC=CC=C5)=O)N6C(C2=C4C(C7=O)=C8C(N7)=O)=C8C9=C6C=CC=C9

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

Stauprimidec-MycAutophagyMycInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Stauprimide
目录号:
HY-N6747
需求量: