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  2. TAT (48-57) TFA

TAT (48-57) TFA 

目录号: HY-P1575A
产品使用指南

TAT (48-57) (TFA) 是可渗透细胞膜的一种多肽,来源于转录蛋白的 HIV-1 反式激活因子 (Tat) 的第 48-57 位氨基酸残基。

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Custom Peptide Synthesis

TAT (48-57) TFA Chemical Structure

TAT (48-57) TFA Chemical Structure

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Size Price Stock
1 mg ¥800 Get quote
5 mg ¥1800 Get quote
10 mg ¥2800 Get quote

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Description

TAT (48-57) (TFA) is a cell-permeable peptide, derived from HIV-1 transactivator of transcription (Tat) protein residue 48-57[1].

Molecular Weight

1510.67

Formula

C₅₅H₁₀₉N₃₁O₁₂.C₂HF₃O₂

Sequence

Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg

Sequence Shortening

GRKKRRQRRR

Shipping

Room temperature in continental US; may vary elsewhere

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
References
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Inquiry Information

产品名称:
TAT (48-57) TFA
目录号:
HY-P1575A
需求量:

TAT (48-57) TFA

Cat. No.: HY-P1575A