1. 诱导疾病模型产品 Neuronal Signaling
  2. 神经系统疾病模型 Amyloid-β
  3. 阿尔兹海默症模型
  4. β-Amyloid (1-42), human, HFIP-treated

β-Amyloid (1-42), human, HFIP-treated 

目录号: HY-P1363B 纯度: 99.89%
COA 产品使用指南 技术支持

β-Amyloid (1-42), human, HFIP-treated,一种 β-Amyloid (1-42), human (HY-P1363A) 经过 HFIP 处理过的由 42 个氨基酸组成的肽,是一种具有脑渗透性的淀粉样蛋白片段,可用于阿尔茨海默病和唐氏综合征的研究。β-Amyloid (1-42), human, HFIP-treated 以单体形式存在时具有抗氧化和神经保护作用。β-Amyloid (1-42), human, HFIP-treated 在经过 DMSO 溶成母液后,一方面在 4 ℃ 孵育形成可溶性寡聚体 (AβOs),具有突触毒性和神经毒性;另一方面,可在 37℃ 孵育形成不溶性纤维,神经毒性较低,参与氧化损伤过程。Aβ42 寡聚体可与多种神经元表面受体 (如 PrPc、mGluR5、NMDA 受体等) 结合,通过激活下游信号通路引发氧化应激、钙稳态失衡和突触毒性,导致神经元功能障碍和死亡。

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Custom Peptide Synthesis

β-Amyloid (1-42), human, HFIP-treated Chemical Structure

β-Amyloid (1-42), human, HFIP-treated Chemical Structure

CAS No. : 107761-42-2

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Other Forms of β-Amyloid (1-42), human, HFIP-treated:

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

β-Amyloid (1-42), human, HFIP-treated, a 42-amino acid peptide that has been treated with HFIP from β-Amyloid (1-42), human (HY-P1363A), is a brain-penetrant amyloid protein fragment, which can be used in research on Alzheimer's disease and Down’s syndrome. β-Amyloid (1-42), human, HFIP-treated remaining as a monomer exhibits antioxidant and neuroprotective effects. β-Amyloid (1-42), human, HFIP-treated, after being dissolved in DMSO to form the stock solution, on the one hand, can form soluble oligomers (AβOs) when incubated at 4 ℃, which have synaptic toxicity and neurotoxicity; on the other hand, it can be incubated at 37 ℃ to form insoluble fibrils, with lower neurotoxicity, and participating in the oxidative damage process. Aβ42 oligomers bind to various neuronal surface receptors (such as PrPc, mGluR5, NMDA receptors, etc.), triggering oxidative stress, calcium homeostasis imbalance, and synaptic toxicity via activating downstream signaling pathways, leading to neuronal dysfunction and death[1][2][3][4][5][6][7].

体外研究
(In Vitro)

β-淀粉样蛋白聚集指南(以下是我们推荐的方案。该方案仅提供指南,应根据您下游实验方案的具体需求进行修改)。
寡聚体制备:
1. 将 β-Amyloid (1-42), human, HFIP-treated 溶解于无水 DMSO 中,浓度为 5 mM,然后涡旋稀释至适当浓度和缓冲液 (冰冷的 PBS/无血清无酚红的 DMEM/F12 培养基) 中。
2. 接下来,将溶液在 4-8 °C 下陈化 24-48 小时。然后将样品在 4-8 °C 下以 14000 g 离心 10 分钟;可溶性低聚物在上清液中。将上清液稀释 10-200 倍用于实验。
纤维体制备:
1. 将 β-Amyloid (1-42), human, HFIP-treated 溶解于无水 DMSO中,浓度为 5 mM,然后用涡旋混合稀释到适当的浓度和缓冲液 (10 mM HCl) 中。
2. 接下来,将溶液在 37 °C 下陈化 24-48 小时,得到 Aβ42 纤维体。
注意:
1) 在进行步骤 1 时,如有少量不溶物,可延长 HFIP 处理时间 (如过夜孵育)、涡旋震荡、或辅助短时间超声;如仍有少量不溶物,建议离心或过滤去除。
2) 聚集形式在溶液中不稳定,建议现配现用。如需储存,建议以薄膜形式的肽储存在 -20 或 -80 °C 下。
β-Amyloid (1-42), human, HFIP-treated 制备成寡聚体,具有神经毒性:
β-amyloid (1-42) peptide (60-180 μM, 48 小时) 形成寡聚体的速度明显快于 Aβ40 和 Aβ43,Aβ42 寡聚体 (1-1000 μg/mL, 48 小时) 在 SH-SY5Y 和 PC12 细胞中表现出最高的神经毒性[10]
Aβ42 oligomers (20 μM, 0-48 小时) 会促使 SH-SY5Y 细胞发生凋亡和自噬,而 U87 细胞则仅发生自噬[11]
Aβ42 oligomers (0.5 μM, 1 小时) 会导致小鼠神经母细胞瘤 N2a 细胞内钙离子内流、线粒体活性氧 (ROS) 生成,并增加细胞死亡率[12]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

4514.04

Formula

C203H311N55O60S

CAS 号
性状

粘稠液体

颜色

Colorless to light yellow

Sequence

Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala

Sequence Shortening

DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA

运输条件

Shipping with dry ice.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料

纯度: 99.89%

参考文献
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  • 稀释计算器

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
β-Amyloid (1-42), human, HFIP-treated
目录号:
HY-P1363B
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