Anti-Mouse CD8a Antibody (53-6.7) 

Anti-Mouse CD8a Antibody (53-6.7) is an anti-mouse CD8a IgG2a antibody inhibitor derived from host Rat. Anti-Mouse CD8a Antibody (53-6.7) binds to CD8αβ stabilizes a conformation with a higher affinity for interaction with MHC class I. Anti-Mouse CD8a Antibody (53-6.7) induces the phosphorylation and activation of TCR proximal signaling pathway components Lck and ZAP70 in polyclonal memory T cells. Anti-Mouse CD8a Antibody (53-6.7) depletes CD8+ T cells and neutralizes cytokine in mice^[1][2][3].

Rat IgG2a kappa

Rat IgG2a kappa, Isotype Control

Mouse

CD8a

Anti-Mouse CD8a Antibody (53-6.7) (4-10 μg) 对表达 CD8αα 的细胞的结合的抑制作用最高可达野生型的 58%^[1]。

Anti-Mouse CD8a Antibody (53-6.7) (1 μg, 30 min) 可以使 COS-7 细胞中 CD8αβ-MHC I 类四聚体相互作用增强^[1]。

Anti-Mouse CD8a Antibody (53-6.7) (10 μg/mL, 15 min) 可诱导多克隆记忆 T 细胞中 T 细胞受体 (TCR) 近端信号通路中 Lck 和 ZAP70 的磷酸化与激活^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse CD8a Antibody (53-6.7) (150-200 μg，腹腔注射，共 4 次) 可以消耗小鼠体内的 CD8+ 细胞^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

12525  [NCBI] & 12526  [NCBI]

P01731

ELISA, FACS, Functional assay, Research in vivo

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

154 kDa

液体

Colorless to light yellow

Shipping with dry ice.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Devine L, et al. Location of the epitope for an anti-CD8alpha antibody 53.6.7 which enhances CD8alpha-MHC class I interaction indicates antibody stabilization of a higher affinity CD8 conformation. Immunol Lett. 2004 May 15;93(2-3):123-30.  [Content Brief]

  [2]. Madi A, et al. CD8 agonism functionally activates memory T cells and enhances antitumor immunity. Int J Cancer. 2022 Sep 1;151(5):797-808.  [Content Brief]

  [3]. Uddin MN, et al. TNF-α-dependent hematopoiesis following Bcl11b deletion in T cells restricts metastatic melanoma. J Immunol. 2014 Feb 15;192(4):1946-53.  [Content Brief]