Phospho-PAK4/5/6 (Ser474/Ser560/Ser602) 抗体 (YA3333)

Phospho-PAK4/5/6 (Ser474/Ser560/Ser602) Antibody (YA3333) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-PAK4/5/6 (Ser474/Ser560/Ser602).

PAK4 是一种丝氨酸/苏氨酸蛋白激酶，在多种信号通路中发挥影响，影响细胞骨架调节、细胞迁移、生长、增殖和细胞存活。由各种效应器（例如生长因子受体或活性 CDC42 和 RAC1）触发的激活会诱导构象变化以及随后多个丝氨酸和/或苏氨酸残基的自磷酸化。PAK4 的磷酸化靶标包括蛋白磷酸酶 SSH1，导致肌动蛋白结合/解聚因子肌动蛋白丝切蛋白的抑制性磷酸化增加。这导致丝切蛋白活性降低，促进肌动蛋白丝的稳定。此外，PAK4 磷酸化 LIMK1，这是另一种抑制丝切蛋白活性的激酶。通过磷酸化整合素 beta5/ITGB5，PAK4 调节细胞运动，而其 ARHGEF2 磷酸化则激活下游靶标 RHOA，从而有助于调节粘着斑组装和肌动蛋白应力纤维。PAK4 通过磷酸化细胞死亡的 BCL2 拮抗剂 BAD，在细胞存活中发挥至关重要的作用。或者，它通过阻止 caspase-8 与死亡结构域受体的结合来抑制细胞凋亡，以激酶独立的方式发挥作用。此外，PAK4 通过控制细胞周期调节蛋白 CDKN1A 的水平和磷酸化 RAN 来影响细胞周期进程。

O96013 / Q9P286 / Q9NQU5

Predicted band size: 64,80,75 kDa; Observed band size: 64,80,75 kDa

Affinity Purified

Non-conjugated

Phosphorylated

AB_3105696

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000

• 
  Western blot analysis of extracts from Hela(lane 2(20ug) , A549(lane 3(20ug) and HL-60(lane 4(20ug) using Phospho-PAK4/5/6 Antibody (HY-P83588) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602)