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  3. Reverse RAS Recruitment System

Reverse RAS Recruitment System反向 Ras 招募系统

释义：

Reverse RAS Recruitment System is used to study interactions between membrane proteins and cytoplasmic proteins, widely applied in signal transduction and cell proliferation research. The bait is a membrane protein (e.g., a receptor or channel protein), and the prey is a fusion protein with a cytoplasmic Ras sequence. When they interact, Ras is recruited to the membrane and activates downstream signaling pathways. The experiment is conducted in yeast strains with temperature-sensitive Ras mutations to prevent spontaneous cell growth.

反向 Ras 招募系统可用于研究膜蛋白和胞质蛋白间的相互作用，广泛应用于信号传导和细胞增殖研究。诱饵为膜蛋白 (如受体或通道蛋白)， 猎物为带有胞质 Ras 序列的融合蛋白。当二者相互作用时，Ras 被招募至膜附近并激活下游信号通路。实验在具有温敏 Ras 突变的酵母菌株中进行，以避免细胞自发性生长。

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