1. GPCR/G Protein Neuronal Signaling
  2. mAChR
  3. DIBA-Cy5

DIBA-Cy5 是一种 DIBA 荧光拮抗剂,由 DIBA-炔结合 Cyanine5 荧光团 (Cy5) 和聚乙二醇 (PEG) 生物分子组成。DIBA-Cy5 可以作为荧光配体,适合通过点击化学附着探针。DIBA-Cy5 对 type-2 mAChR (M2R) 具有很高的结合亲和力 ( Kd=1.80 nM),可直接染色小鼠窦房结的 M2R 受体。

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DIBA-Cy5 Chemical Structure

DIBA-Cy5 Chemical Structure

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Top Publications Citing Use of Products
  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

DIBA-Cy5 is a fluorescent DIBA antagonist made up be DIBA-alkyne binding Cyanine5 fluorophores (Cy5) and polyethylene glycol (PEG) biomolecules. DIBA-Cy5 can serve as a fluorescent ligand, suitable for probe attachment through click chemistry. DIBA-Cy5 exerts a high binding affinity to type-2 mAChR (M2R) with the Kd value of 1.80 nM, can directly stain M2R receptors in the sinoatrial node of a mouse heart[1].

IC50 & Target[1]

mAChR2

1.80 nM (Kd)

mAChR1

104.5 nM (Kd)

体外研究
(In Vitro)

DIBA-Cy5 (0.01 nM-1 mM; 5 h) shows selective binding property for type-2 mAChR (M2R) over M1R, with Kd of 1.08 nM and 104.5 nM, respectively[1].
DIBA-Cy5 (10 nM; 3 h) exerts the competitive binding mode with orthosteric antagonist Atropine (HY-B1205)/allosteric modulator LY2119620 (HY-15885), indicating a dualsteric binding mode of the DIBA-type antagonist to M2R[1].
DIBA-Cy5 (50 nM; 16 h) results direct staining to M2R receptors in the sinoatrial node of a mouse heart. DIBA-Cy5 can be used binding assays that screen compounds for M2R as the receptor target[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1360.71

Formula

C69H89N11O12S3

Emission (Em)

668

Excitation (Ex)

647

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献

DIBA-Cy5 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
DIBA-Cy5
目录号:
HY-151801
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