2. NF-κB MAPK/ERK Pathway Apoptosis
  3. NF-κB p38 MAPK Bcl-2 Family Caspase Apoptosis
  4. DNH28

DNH28 是一种强效的 NF-κB 和 MAPK 抑制剂，对 HepG2 细胞的 IC50 值为 0.93 μM。DNH28 通过下调 Bcl-2，上调 BAX 和 Cleaved-caspase-3 的表达，诱导细胞凋亡 (apoptosis)。DNH28 可阻滞细胞周期，抑制细胞迁移。DNH28 可用于肝细胞癌 (HCC) 的研究。

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DNH28

DNH28 Chemical Structure

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DNH28 相关抗体

Top Publications Citing Use of Products

查看 NF-κB 亚型特异性产品:

查看所有亚型
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

查看 p38 MAPK 亚型特异性产品:

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p38 MAPK p38α p38β MAPK13 p38δ p38γ

查看 Bcl-2 Family 亚型特异性产品:

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Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3

查看 Caspase 亚型特异性产品:

查看所有亚型
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

DNH28 is a potent NF-κB and MAPK inhibitor with an IC50 of 0.93 μM against HepG2 cells. DNH28 promotes apoptosis by down-regulating the expression of Bcl-2, up-regulating the expression of BAX and Cleaved-caspase-3. DNH28 blocks the cell cycle and inhibits migration. DNH28 can be used for the study of hepatocellular carcinoma (HCC)[1].

IC50 & Target[1]

Caspase 3

 

Bax

 

Bcl-2

 

NF-κB

 

体外研究
(In Vitro)

DNH28 (Compound 28) 对正常细胞 THLE-3 的毒性较低 (IC50 = 18.16 μM) [1]。
DNH28 (0.46-1.84 μM, 24 h) 通过诱导细胞凋亡来抑制 HepG2 细胞的增殖，并且剂量依赖性使得 HepG2 细胞停滞于 G2/M 期[1]。
DNH28 (0.46-1.84 μM, 24 h) 能剂量依赖性地抑制 HepG2 细胞的迁移，并且在 1.84 μM 时，能完全抑制其迁移能力[1]。
DNH28 (1.84 μM, 24 h) 有效地抑制了由 TNF-α 引发的 p65 的核转移[1]。
DNH28 (0.46-1.84 μM, 24 h) 通过抑制 NF-κB 和 MAPK 信号通路的激活来发挥抗肝细胞癌的作用[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

DNH28 相关抗体:

Apoptosis Analysis[1]

Cell Line: HepG2 cells
Concentration: 0.46, 0.92 and 1.84 μM
Incubation Time: 24 h
Result: Induced apoptosis dose-dependently.
Reached 45.90% of apoptotic cells at 1.84 μM (compared with 14.57% in the control group).

Cell Cycle Analysis[1]

Cell Line: HepG2 cells
Concentration: 0.46, 0.92 and 1.84 μM
Incubation Time: 24 h
Result: Reached 76.22% G2/M phase ratio at 1.84 μM.

Cell Migration Assay [1]

Cell Line: HepG2 cells
Concentration: 0.46, 0.92 and 1.84 μM
Incubation Time: 24 h
Result: Partially suppressed cell migration, whereas at 1.84 μM, the scratch width remained unchanged.

RT-PCR[1]

Cell Line: HepG2 cells
Concentration: 0.46, 0.92 and 1.84 μM
Incubation Time: 24 h
Result: Increased the expression of pro-apoptotic mRNA levels BAX and C-caspase-3.
Reduced the expression of the anti-apoptotic mRNA levels of BCL-2.

Western Blot Analysis[1]

Cell Line: HepG2 cells
Concentration: 0.46, 0.92 and 1.84 μM
Incubation Time: 24 h
Result: Exhibited dose-dependent inhibition of the phosphorylation of p65, IκB-α, p38 and ERK.
Significantly down-regulated the activation of the NF-κB and MAPK signaling pathways
分子量

436.90

Formula

C23H24ClF3N2O
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

DNH28 相关分类

  • 摩尔计算器

  • 稀释计算器

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DNH28DNH 28DNH-28NF-κBp38 MAPKBcl-2 FamilyCaspaseApoptosisNuclear factor-κBNuclear factor-kappaBAnti-HCCNF-κB inhibitorMAPK inhibitorNF-κB pathwayMAPK pathwayHepG2 cellsInhibitorinhibitorinhibit

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