GRGDSPK TFA  (Synonyms: EMD 56574 TFA)

GRGDSPK TFA (EMD 56574 TFA) is a peptide incluing Arg-Gly-Asp (RGD). GRGDSPK TFA is an competitive and reversible inhibitory peptide for inhibiting integrin-fibronectin binding. GRGDSPK TFA is used to study the role of integrins in bone formation and resorption^[1][2].

GRGDSPK TFA (EMD 56574 TFA; RGD; 0.1-50 μM; for 4 days) inhibits mineralization in a dose-dependent manner as determined by measuring calcium content and 70 bonehnit area of tissue in parietal bones 18 days old isolated from pregnant Sprague-Dawley rats^[1].
GRGDSPK TFA (10, 50 μM; for 4 days) dramatically alteres bone morphology, with a disruption of osteoblast and mineralized matrix organization^[1].
GRGDSPK TFA (RGD; 250 μM), added to the medium, effectively blocks integrin-fibronectin binding and significantly increases the average size of wild-type cell aggregates^[2].
When GRGDSPK TFA (250 μM) is added, wild-type mesendodermal progenitors exhibits strongly reduced adhesion forces and work, indicating that the detachment parameters recorded are specific for fibronectin and that integrins expressed in mesendodermal progenitors are involved^[2].
GRGDSPK TFA (RGD-containing, 1.5 mM, 1.0 mM, and 0.5 mM) and RGD-mod-ified peptides impair the ability of sperm to fertilizebovine oocytes, illustrated by a reduction in cleavage in a dose-dependent manner^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

829.78

C₃₀H₅₀F₃N₁₁O₁₃

Gly-Arg-Gly-Asp-Ser-Pro-Lys

GRGDSPK

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. G A Gronowicz, et al. Synthetic Peptide Containing Arg-Gly-Asp Inhibits Bone Formation and Resorption in a Mineralizing Organ Culture System of Fetal Rat Parietal Bones. J Bone Miner Res. 1994 Feb;9(2):193-201.  [Content Brief]

  [2]. Pierre-Henri Puech, et al. Measuring Cell Adhesion Forces of Primary Gastrulating Cells From Zebrafish Using Atomic Force Microscopy. J Cell Sci. 2005 Sep 15;118(Pt 18):4199-206.  [Content Brief]

  [3]. Sessions BR, et al. Effects of amino acid substitutions in and around the arginine-glycine-aspartic acid (RGD) sequence on fertilization and parthenogenetic development in mature bovine oocytes. Mol Reprod Dev. 2006 May;73(5):651-7.  [Content Brief]