2. Academic Validation
  3. Identification and Analysis of Amygdalin, Neoamygdalin and Amygdalin Amide in Different Processed Bitter Almonds by HPLC-ESI-MS/MS and HPLC-DAD

Identification and Analysis of Amygdalin, Neoamygdalin and Amygdalin Amide in Different Processed Bitter Almonds by HPLC-ESI-MS/MS and HPLC-DAD

  • Molecules. 2017 Aug 30;22(9):1425. doi: 10.3390/molecules22091425.
Shuya Xu 1,  Xinfang Xu 2,  Shaoxiong Yuan 3,  Huan Liu 4,  Mengnan Liu 5,  Ying Zhang 6,  Hui Zhang 7,  Yan Gao 8,  Ruichao Lin 9,  Xiangri Li 10
Affiliations

Affiliations

  • 1 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. xushuya@bucm.edu.cn.
  • 2 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. xuxinfang007@163.com.
  • 3 Marketing Department, Leading Pharm Medical Technology, Inc., Beijing 100083, China. shaoxiongyuan@126.com.
  • 4 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. 20150931937@bucm.edu.cn.
  • 5 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. 20160931810@bucm.edu.cn.
  • 6 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. zhang0312ying@163.com.
  • 7 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. zh19930503@sina.com.
  • 8 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. 20150931752@bucm.edu.cn.
  • 9 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. linrch307@sina.com.
  • 10 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. lixiangri@sina.com.
PMID: 28867779 DOI: 10.3390/molecules22091425
Abstract

Processing is a traditional pharmacy technology based on traditional Chinese medicine theory. The traditional Chinese medicine (TCM) ingredients should be processed before being used as a medicine. Processed bitter almonds are widely used in the clinic in TCM for the treatment of cough and asthma. In this work the amygdalin profile of three producing areas in China was determined, with respect to three differently processed bitter almond products: raw, stir-fried and scalded. Identification of the compounds was done by using high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS/MS). Results indicated that amygdalin, neoamygdalin and amygdalin amide were identified in the different processed bitter almonds. Meanwhile, amygdalin was used as a standard to calculate the quantification of amygdalin and the concentration ratio of neoamygdalin and total amygdalin by HPLC-DAD. The data suggested that composition of amygdalin isomers in bitter almonds was influenced by the processing method. It also gives a new understanding of the processing principle of bitter almonds. Moreover, the classification of different processed bitter almonds can be achieved on the basis of amygdalin isomers levels.

Keywords

HPLC-ESI-MS/MS; almonds; amygdalin; neoamygdalin; processing.

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