1. Academic Validation
  2. Specific PKC βII inhibitor: one stone two birds in the treatment of diabetic foot ulcers

Specific PKC βII inhibitor: one stone two birds in the treatment of diabetic foot ulcers

  • Biosci Rep. 2018 Sep 7;38(5):BSR20171459. doi: 10.1042/BSR20171459.
Sushant Kumar Das 1 2 Yi Feng Yuan 1 Mao Quan Li 3
Affiliations

Affiliations

  • 1 Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University, 301 Yanchang Road, Shanghai 200072, China.
  • 2 Department of Interventional Radiology, Affiliated Hospital of North Sichuan Medical College, 63 Wenhua Road, Nanchong, Sichuan 637000, China.
  • 3 Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University, 301 Yanchang Road, Shanghai 200072, China 782616733@qq.com.
Abstract

To explore whether or not inhibition of protein kinase C βII (PKC βII) stimulates angiogenesis as well as prevents excessive NETosis in diabetics thus accelerating wound healing. Streptozotocin (STZ, 60 mg/kg/day for 5 days, i.p.) was injected to induce type I diabetes in male ICR mice. Mice were treated with ruboxistaurin (30 mg/kg/day, orally) for 14 consecutive days. Wound closure was evaluated by wound area and number of CD31-stained capillaries. Peripheral blood flow cytometry was done to evaluate number of circulating endothelial progenitor cells (EPCs). NETosis assay and wound tissue immunofluorescence imaging were done to evaluate the percentage of neutrophils undergoing NETosis. Furthermore, the expression of PKC βII, protein kinase B (Akt), endothelial nitric oxide synthase (eNOS), vascular endothelial growth factor (VEGF), and histone citrullation (H3Cit) were determined in the wound by Western blot analysis. Ruboxistaurin accelerated wound closure and stimulated angiogenesis in diabetic mice. The number of circulating EPCs was increased significantly in ruboxistaurin-treated diabetic mice. Moreover, ruboxistaurin treatment significantly decreases the percentages of H3Cit+ cells in both peripheral blood and wound areas. This prevented excess activated neutrophils forming an extracellular trap (NETs) formation. The expressions of phospho-Akt (p-Akt), phospho-eNOS (p-eNOS), and VEGF increased significantly in diabetic mice on ruboxistaurin treatment. The expressions of PKC βII and H3Cit+, on the other hand, decreased with ruboxistaurin treatment. The results of the present study suggest that ruboxistaurin by inhibiting PKC βII activation, reverses EPCs dysfunction as well as prevents exaggerated NETs formation in a diabetic mouse model; thereby accelerating the wound healing process.

Keywords

NETosis; Protein Kinase C; angiogenesis; diabetic foot; neutrophil extracellular traps.

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