2. Academic Validation
  3. Developmental toxicity of 2-bromoacetamide on peri- and early post-implantation mouse embryos in vitro

Developmental toxicity of 2-bromoacetamide on peri- and early post-implantation mouse embryos in vitro

  • Ecotoxicol Environ Saf. 2023 Mar 1:252:114612. doi: 10.1016/j.ecoenv.2023.114612.
Chuanxin Zhang 1,  Xueqi Dong 1,  Xiaoyu Yin 1,  Xinyi Yuan 1,  Jiawei Wang 1,  Jinzhu Song 1,  Zhenzhen Hou 1,  Cheng Li 1,  Keliang Wu 2
Affiliations

Affiliations

  • 1 Center for Reproductive Medicine, Shandong University, Jinan, Shandong 250012, China; Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong 250012, China; Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong 250012, China; Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong 250012, China; Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong 250012, China; National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong 250012, China.
  • 2 Center for Reproductive Medicine, Shandong University, Jinan, Shandong 250012, China; Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong 250012, China; Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong 250012, China; Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong 250012, China; Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong 250012, China; National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong 250012, China. Electronic address: wukeliang_527@163.com.
PMID: 36774798 DOI: 10.1016/j.ecoenv.2023.114612
Abstract

2-bromoacetamide (BAcAm), a new class of disinfection by-products (DBPs), is widely detected in drinking water across the world. Reports of the high cytogenetic toxicity of BAcAm have aroused public attention concerning its toxic effects on early embryonic development. In this study, we optimized an in vitro culture (IVC) system for peri- and early post-implantation mouse embryos and used this system to determine the developmental toxicity of BAcAm. We found that exposure to BAcAm caused a reduction in egg cylinder formation rate and abnormal lineage differentiation in a dose-dependent manner. Transcriptomic analysis further revealed that BAcAm exposure at early developmental stages altered the abundance of transcripts related to a variety of biological processes including gene expression, metabolism, cell proliferation, cell death and embryonic development, thus indicating its toxic effects on embryonic development. Thus, we developed a robust tool for studying the toxicology of chemicals at the early stages of embryonic development and demonstrated the developmental toxicity of BAcAm in the early embryonic development of mammals.

Keywords

2-bromoacetamide; Developmental toxicity; In vitro culture; Mouse embryo; Single-cell RNA-sequencing.

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