1. Academic Validation
  2. IGF-PLGA microspheres promote angiogenesis and accelerate skin flap repair and healing by inhibiting oxidative stress and regulating the Ang 1/Tie 2 signaling pathway

IGF-PLGA microspheres promote angiogenesis and accelerate skin flap repair and healing by inhibiting oxidative stress and regulating the Ang 1/Tie 2 signaling pathway

  • Eur J Pharm Sci. 2024 Jan 2:193:106687. doi: 10.1016/j.ejps.2023.106687.
Fei Hu 1 Kai Huang 2 Hanbo Zhang 1 Wenjie Hu 2 Songlin Tong 2 Hongming Xu 3
Affiliations

Affiliations

  • 1 Cixi Biomedical Research Institute, Wenzhou Medical University, Cixi, Ningbo, China.
  • 2 Department of Orthopaedic Surgery, Affiliated Cixi Hospital, Wenzhou Medical University, No. 999, South Second Ring Road, Hushan Street, Cixi, Ningbo 315300, China.
  • 3 Department of Orthopaedic Surgery, Affiliated Cixi Hospital, Wenzhou Medical University, No. 999, South Second Ring Road, Hushan Street, Cixi, Ningbo 315300, China. Electronic address: 104497554@qq.com.
Abstract

Random flaps are widely used in the treatment of injuries, tumors, congenital malformations, and Other Diseases. However, postoperative skin flaps are prone to ischemic necrosis, leading to surgical failure. Insulin-like growth factor- 1(IGF-1) belongs to the IGF family and exerts its growth-promoting effects in various tissues through autocrine or paracrine mechanisms. Its application in skin flaps and other traumatic diseases is relatively limited. Poly (lactic-co-glycolic acid) (PLGA) is a degradable high-molecular-weight organic compound commonly used in biomaterials. This study prepared IGF-PLGA sustained-release microspheres to explore their impact on the survival rate of flaps both in vitro and in vivo, as well as the mechanisms involved. The research results demonstrate that IGF-PLGA has a good sustained-release effect. At the cellular level, it can promote 3T3 cell proliferation by inhibiting oxidative stress, inhibit Apoptosis, and enhance the tube formation ability of human umbilical vein endothelial cells (HUVEC) . At the animal level, it accelerates FLAP healing by promoting vascularization through the inhibition of oxidative stress. Furthermore, this study reveals the role of IGF-PLGA in activating the Angiopoietin-1(Ang1)/Tie2 signaling pathway in promoting FLAP vascularization, providing a strong theoretical basis and therapeutic target for the application of IGF-1 in flaps and other traumatic diseases.

Keywords

Angiogenesis; Flap; IGF-PLGA; Microspheres; Oxidative stress.

Figures
Products
Inhibitors & Agonists
Other Products