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  3. A fungal chromatin remodeler drives transcription by establishing an open chromatin architecture and activated histone environment

A fungal chromatin remodeler drives transcription by establishing an open chromatin architecture and activated histone environment

  • Cell Rep. 2025 Aug 20;44(9):116169. doi: 10.1016/j.celrep.2025.116169.
Mengting Xu 1,  Qi Zhang 1,  Yuan Li 1,  Wei Zhou 1,  Lingyun Lei 1,  Fucheng Lin 2,  Yanjun Kou 3,  Zeng Tao 4
Affiliations

Affiliations

  • 1 State Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Provincial Key Laboratory of Agricultural Microbiomics, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
  • 2 State Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Provincial Key Laboratory of Agricultural Microbiomics, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China; State Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Provincial Key Laboratory of Agricultural Microbiomics, Key Laboratory of Agricultural Microbiome, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
  • 3 State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • 4 State Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Provincial Key Laboratory of Agricultural Microbiomics, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. Electronic address: taozeng@zju.edu.cn.
PMID: 40844877 DOI: 10.1016/j.celrep.2025.116169
Abstract

Chromatin remodelers are central regulators of chromatin architecture and transcriptional dynamics, yet the mechanisms underlying the establishment of transcriptionally permissive chromatin and an activated histone environment remain elusive. In the filamentous fungus Magnaporthe oryzae, we demonstrate that remodeling the structure of chromatin 1 (RSC1) functions as a critical regulator in this process. RSC1 is indispensable for establishing open chromatin architecture, and its activity is tightly correlated with altered nucleosome occupancy. Mechanistically, RSC1 physically associates with a H3K27me3 demethylase and positively modulates its abundance, thereby establishing precise chromatin boundaries to restrict heterochromatin spreading. Additionally, RSC1 recognizes histone acetylation and recruits Histone Acetyltransferase, which synergistically drive histone hyperacetylation at its targets. By integrating a chromatin remodeling complex, H3K27 demethylation, and histone hyperacetylation, RSC1 orchestrates an activated histone environment to promote transcriptional permissiveness. Collectively, these findings elucidate pivotal roles of RSC1 in counteracting facultative heterochromatin formation, enhancing chromatin accessibility, and activating gene expression in fungi.

Keywords

CP: Molecular biology; Chromatin remodelers; H3K27 demethylation; Magnaporthe oryzae; RSC1; chromatin accessibility; histone acetylation; transcriptional activation.

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