2. Academic Validation
  3. m6A-RNA epitranscriptomes regulate splicing and neuronal development in the Pacific oyster Crassostrea gigas

m6A-RNA epitranscriptomes regulate splicing and neuronal development in the Pacific oyster Crassostrea gigas

  • Genomics. 2025 Oct 13;117(6):111142. doi: 10.1016/j.ygeno.2025.111142.
Natacha Clairet 1,  Hélène Auger 2,  Roberto-Carlos Arredondo-Espinoza 3,  Hugo Koechlin 4,  Benoît Bernay 5,  Lukas Manoury 6,  Didier Goux 7,  Guillaume Rivière 8
Affiliations

Affiliations

  • 1 UR7482 MERSEA, Marine Ecosystems and Organisms Research Laboratory, Université de Caen Normandie, UNICAEN, 14032 Caen, France; Normandy University, Caen, France. Electronic address: natacha.clairet@unicaen.fr.
  • 2 UR7482 MERSEA, Marine Ecosystems and Organisms Research Laboratory, Université de Caen Normandie, UNICAEN, 14032 Caen, France; Normandy University, Caen, France. Electronic address: helene.auger@unicaen.fr.
  • 3 UR7482 MERSEA, Marine Ecosystems and Organisms Research Laboratory, Université de Caen Normandie, UNICAEN, 14032 Caen, France; Normandy University, Caen, France. Electronic address: Roberto.arredondo@satmar.fr.
  • 4 Ifremer, Univ Brest, CNRS, IRD, LEMAR, IUEM, Plouzané, France. Electronic address: Hugo.Koechlin@ifremer.fr.
  • 5 Normandy University, Caen, France; UNICAEN, Université de Caen Normandie, SF MerLIN, US EMERODE, Plateforme Proteogen, Caen, France. Electronic address: benoit.bernay@unicaen.fr.
  • 6 Normandy University, Caen, France.
  • 7 Normandy University, Caen, France; UNICAEN, Université de Caen Normandie, SF MerLIN, US EMERODE, CMAbio3: Centre de Microscopie Appliquée à la Biologie, Caen, France. Electronic address: Didier.goux@unicaen.fr.
  • 8 UR7482 MERSEA, Marine Ecosystems and Organisms Research Laboratory, Université de Caen Normandie, UNICAEN, 14032 Caen, France; Normandy University, Caen, France. Electronic address: guillaume.riviere@unicaen.fr.
PMID: 41093079 DOI: 10.1016/j.ygeno.2025.111142
Abstract

N6-adenosine RNA methylation (m6A) is a key regulator of gene expression during embryogenesis and neurogenesis in mammals and insects. However, its functional relevance remains unknown in lophotrochozoans like the Pacific oyster Crassostrea gigas, despite its association with developmental gene expression. We treated oyster embryos with STM2457, a METTL3 methyltransferase-inhibitor. m6A-RNA reduction in treated embryos induced morphological alterations and 5-HT immunohistochemistry revealed impaired neuronal development. Transcriptome and proteome analyses indicated that m6A inhibition disturbs transcription and translation. Epitranscriptome Sequencing showed that m6A inhibition increased transcript length by exon and intron retention, suggesting m6A-dependent recruitment of splicing factors at intron-exon boundaries. Together, our results support an essential role for m6A in neural differentiation and development in the oyster by regulating alternative splicing. This study provides the first evidence of a functional role for m6A in lophotrochozoan development, providing new eco-evo-devo insights of epitranscriptomic processes.

Keywords

Alternative splicing; Embryonic development; Epitranscriptomics; Molluscs; Proteome; STM2457; Transcriptome.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z