1. Academic Validation
  2. Cloning and sequencing of an ovine interleukin-5 cDNA

Cloning and sequencing of an ovine interleukin-5 cDNA

  • DNA Seq. 1996;6(6):331-5. doi: 10.3109/10425179609047571.
H F Seow 1 M J David P McWaters L Hurst P R Wood
Affiliations

Affiliation

  • 1 CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria, Australia. heng@burnet.mbcmr.unimelb.edu.au
Abstract

Interleukin-5 (IL-5) is a T-cell derived cytokine which stimulates eosinophil production and activation in human, mice and sheep. IL-5 is active as a growth factor for mouse but not human B cells. The role of IL-5 on ruminant B cells has not been clearly defined. By hybridisation with human IL-5 cDNA, the ovine IL-5 gene was isolated from a liver genomic library. The IL-5 cDNA was obtained by reverse-transcriptase PCR using primers designed from the 5' and 3' coding sequence derived from the ovine IL-5 gene. The sequences of the cDNA shows that there is 79% and 73% nucleotide homology with the human and mouse sequences. The ovine IL-5 cDNA encoded a protein of 132 Amino acids and the level of amino acid homology with human and mouse IL-5 is 64% and 56%, respectively. Two cysteine residues are conserved in ovine, human and mouse IL-5. There are two potential N-linked glycoyslation sites in ovine IL-5.

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