2. Epigenetics PI3K/Akt/mTOR MAPK/ERK Pathway Stem Cell/Wnt Metabolic Enzyme/Protease Immunology/Inflammation NF-κB GPCR/G Protein
  3. AMPK mTOR ERK Oxidative Phosphorylation Reactive Oxygen Species (ROS) Ras
  4. NMac1

NMac1 是一种具有口服活性的 Nm23/NDPK 激活剂。NMac1 直接与 Nm23-H1 结合并激活重组 Nm23-H1 的 NDPK 活性，EC50 为 10.7 uM。NMac1 在 MDA-MB-231 细胞中诱导 AMPK 活化并抑制 mTOR 和 ERK，诱导线粒体 OXPHOS 失调，抑制线粒体 ROS 进而诱导线粒体功能障碍。NMac1 抑制 MDA-MB-231 细胞中的复合物 I 活性，抑制 Rac1 活化后形态学和肌动蛋白细胞骨架组织的变化。NMac1 抑制肿瘤侵袭，迁移以及转移。NMac1 可用于转移性肿瘤如乳腺癌的研究。NMac1 可以从姜 cassumunar Roxb 中分离出。

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NMac1

NMac1 Chemical Structure

CAS No. : 1332290-68-2

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Customer Review

Other Forms of NMac1:

NMac1 相关抗体

Top Publications Citing Use of Products

查看 AMPK 亚型特异性产品:

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NUAK1 NUAK2 AMPK AMPK α1β1γ1 AMPK α2β1γ1 AMPK α1β2γ1 AMPK α2β2γ1 BRSK1 BRSK2 HUNK AMPKα

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ERK ERK1 ERK2 ERK5 ERK8

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K-Ras H-Ras N-Ras Ras

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

NMac1 is an orally active Nm23/NDPK activator. NMac1 directly binds to Nm23-H1 and activates the NDPK activity of recombinant Nm23-H1 with an EC50 of 10.7 uM. NMac1 induces AMPK activation and inhibits mTOR and ERK, leading to mitochondrial OXPHOS dysregulation and suppressing mitochondrial ROS production, which in turn induces mitochondrial dysfunction in MDA-MB-231 cells. NMac1 inhibits Complex I activity and suppresses changes in morphology and actin cytoskeleton organization following Rac1 activation in MDA-MB-231 cells. NMac1 inhibits tumor invasion, migration and metastasis. NMac1 is useful for studying metastatic tumors, such as breast cancer. NMac1 can be isolated from the ginger cassumunar Roxb[1][2].

体外研究
(In Vitro)

NMac1 抑制葡萄糖饥饿条件下 MDA-MB-231 和 MCF7 细胞中癌细胞的增殖 (IC50s 分别为 7.197 和 2.849 μM)[1]。
NMac1 (0-20 μM，0-20 小时) 在缺乏葡萄糖的 MDA-MB-231 细胞中诱导 AMPK 活化并抑制 mTOR 和 ERK[1]。
NMac1 (0-20 μM，0-20 小时) 通过抑制 ATP 产生并降低 MDA-MB-231 细胞中的线粒体膜电位来诱导线粒体功能障碍[1]。
NMac1 (0-10 μM，0-100 分钟) 在 MDA-MB-231 细胞中，在缺乏葡萄糖的情况下，通过降低氧消耗率来诱导 ATP 耗竭，抑制线粒体 ROS，并诱导线粒体 ATP 生成系统 OXPHOS 失调[1]。
NMac1 (250-500 μM，0-100分钟) 可抑制 MDA-MB-231 细胞中的复合物 I 活性[1]。
NMac1 (0-30 μM，2-14天) 以剂量依赖性方式抑制 MDA-MB-231 细胞球体增殖[1]。
NMac1 (0-25 μM，16-24小时) 通过 NDPK 激活 Nm23-H1 来抑制 Rac1 的活化，从而降低 MDA-MB-231 细胞的侵袭和迁移[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

NMac1 相关抗体:

Western Blot Analysis[1]

Cell Line: MDA-MB-231 cells
Concentration: 0, 5, 10, 12.5, 20 μM
Incubation Time: 0, 2, 4, 8, 12, 16, 20 h
Result: Induced AMPK activation and inhibited mTOR, ERK, and pS6K, rapidly induces AMPK activation within 2 hours under glucose depletion.

Immunofluorescence[1]

Cell Line: MDA-MB-231 cells
Concentration: 12.5 μM
Incubation Time: 6 h
Result: Deteriorated the mitochondrial integrity of cells in the absence of glucose, reduced TOM20 levels.

Western Blot Analysis[2]

Cell Line: MDA-MB-231 cells
Concentration: 0, 5, 10, 25 μM
Incubation Time: 16 h
Result: Reduced Rac1 activation.

Cell Migration Assay [2]

Cell Line: MDA-MB-231 cells
Concentration: 0, 5, 10, 25 μM
Incubation Time: 24 h
Result: Reduced the invasion and migration in a dose dependent manner, without affecting their proliferation.
体内研究
(In Vivo)

NMac1 (10 mg/kg，口服，每日一次，21 天) 在乳腺癌转移小鼠模型中可抑制乳腺癌转移，且不会影响原发性肿瘤大小[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Breast cancer (MDA-MB-231-Luc-D3H2LN cells, 1 × 106) metastasis NOD/SCID mice model[2]
Dosage: 10 mg/kg
Administration: p.o., once a day, 21 days
Result: Significantly inhibited breast cancer metastasis without affecting primary tumor size.
分子量

380.48

Formula

C24H28O4
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

NMac1 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NMac11332290-68-2NMac 1NMac-1AMPKmTORERKOxidative PhosphorylationReactive Oxygen Species (ROS)RasAMP-activated protein kinaseMammalian target of RapamycinExtracellular signal regulated kinasesActivatorMDA-MB-231 cellsMCF7 cellsNOD/SCID miceInhibitorinhibitorinhibit

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