1. MAPK/ERK Pathway Stem Cell/Wnt JAK/STAT Signaling Protein Tyrosine Kinase/RTK Metabolic Enzyme/Protease Immunology/Inflammation NF-κB Neuronal Signaling Membrane Transporter/Ion Channel
  2. ERK EGFR Reactive Oxygen Species Calcium Channel
  3. PACAP-38 (31-38), human, mouse, rat TFA

PACAP-38 (31-38), human, mouse, rat TFA 

目录号: HY-P1845A 纯度: 99.59%
COA 产品使用指南

PACAP-38 (31-38), human, mouse, rat TFA 是一种 PAC1 受体激活剂。PACAP-38 (31-38), human, mouse, rat TFA 增加 α-分泌酶活性。PACAP-38 (31-38), human, mouse, rat 升高胞浆 Ca2+,增加细胞外调节激酶 (ERK) 和表皮生长因子受体 (EGFR) 的增殖和磷酸化。PACAP-38 (31-38), human, mouse, rat TFA 对交感神经 NPY 和儿茶酚胺的产生具有强效、持续的刺激作用。PACAP-38 (31-38), human, mouse, rat TFA 可用于神经营养和神经保护研究。

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Custom Peptide Synthesis

PACAP-38 (31-38), human, mouse, rat TFA Chemical Structure

PACAP-38 (31-38), human, mouse, rat TFA Chemical Structure

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2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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1 mg ¥1500
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5 mg ¥4500
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10 mg ¥6700
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100 mg   询价  

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Other Forms of PACAP-38 (31-38), human, mouse, rat TFA:

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Top Publications Citing Use of Products
  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

PACAP-38 (31-38), human, mouse, rat TFA is a PAC1 receptor activator and increases the α-secretase activity. PACAP-38 (31-38), human, mouse, rat TFA elevates cytosolic Ca2+, increases proliferation and increases phosphorylation of extracellular regulates kinase (ERK) and the epidermal growth factor receptor (EGFR). PACAP-38 (31-38), human, mouse, rat TFA demonstrates potent, efficacious, and sustained stimulatory effects on sympathetic neuronal NPY and catecholamine production. PACAP-38 (31-38), human, mouse, rat TFA can be used for neurotrophic and neuroprotective research[1][2][3].

体外研究
(In Vitro)

PACAP-38 (31-38), human, mouse, rat TFA (100 nM; 2 min; NCI-H838 cells) induces EGFR, HER2 and ERK tyrosine phosphorylation[1].
PACAP-38 (31-38), human, mouse, rat TFA (100 nM; 30 min; NCI-H838 cells) induces EGFR tyrosine phosphorylation with generates ROS and increases ROS levels by 51%[1].
PACAP-38 (31-38), human, mouse, rat TFA (10 nM; 48 h) stimulates the growth of NCI-H838 cells[1].
PACAP-38 (31-38), human, mouse, rat TFA (300 nM; 4 h) stimulates generation of APPsα in neural cells[2].
PACAP-38 (31-38), human, mouse, rat TFA (0.01-10 nM; HEK 293 cells) stimulates neural cells express endogenous PAC1 receptors by cAMP accumulation and by an increase in cytosolic free calcium and induces elevation of the intracellular Ca2+ concentration in a dose-dependent manner with an EC50 value of 0.81 nM[2].
PACAP-38 (31-38), human, mouse, rat TFA (0.01 nM; 48 h) elicits potent and efficacious stimulation of NPY secretion from SCG neuronal cultures[3].
PACAP-38 (31-38), human, mouse, rat TFA (100 nM; 14 d) produces sustained stimulated NPY and catecholamine secretionmpathetic neurons[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: NCI-H838 cells
Concentration: 10 nM
Incubation Time: 48 hours
Result: Increased the number of NCI-H838 cells by 72%.

Western Blot Analysis[1]

Cell Line: NCI-H838 cells
Concentration: 100 nM
Incubation Time: 2 min
Result: Increased tyrosine phosphorylation of the EGFR, HER2, and ERK by 377, 299 and 216%, respectively.
分子量

1176.29

Formula

C49H84F3N17O13

性状

固体

颜色

White to off-white

Sequence

Tyr-Lys-Gln-Arg-Val-Lys-Asn-Lys-NH2

Sequence Shortening

YKQRVKNK-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O 中的溶解度 : 100 mg/mL (85.01 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.8501 mL 4.2507 mL 8.5013 mL
5 mM 0.1700 mL 0.8501 mL 1.7003 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

In Vivo:

以下溶解方案,请直接配置工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

  • 方案 一

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (85.01 mM); 澄清溶液; 超声助溶

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
纯度 & 产品资料
参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.8501 mL 4.2507 mL 8.5013 mL 21.2533 mL
5 mM 0.1700 mL 0.8501 mL 1.7003 mL 4.2507 mL
10 mM 0.0850 mL 0.4251 mL 0.8501 mL 2.1253 mL
15 mM 0.0567 mL 0.2834 mL 0.5668 mL 1.4169 mL
20 mM 0.0425 mL 0.2125 mL 0.4251 mL 1.0627 mL
25 mM 0.0340 mL 0.1700 mL 0.3401 mL 0.8501 mL
30 mM 0.0283 mL 0.1417 mL 0.2834 mL 0.7084 mL
40 mM 0.0213 mL 0.1063 mL 0.2125 mL 0.5313 mL
50 mM 0.0170 mL 0.0850 mL 0.1700 mL 0.4251 mL
60 mM 0.0142 mL 0.0708 mL 0.1417 mL 0.3542 mL
80 mM 0.0106 mL 0.0531 mL 0.1063 mL 0.2657 mL

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
PACAP-38 (31-38), human, mouse, rat TFA
目录号:
HY-P1845A
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