2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. 15-PGDH/HPGD 蛋白, Human (HEK293, His)

15-PGDH/HPGD 蛋白, Human (HEK293, His)

目录号: HY-P70297
COA 产品使用指南

15-PGDH/HPGD 蛋白, Human (HEK 293, His) 是在 HEK293 细胞中表达，N 端带有一个 His 标签的重组人 15-PGDH/HPGD。15-PGDH/HPGD 是降解前列腺素的关键酶，在炎症相关疾病的发生发展中起重要作用。

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规格 价格 是否有货 数量
10 μg ￥1600 In-stock
50 μg ￥4500 In-stock
100 μg   询价  

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Top Publications Citing Use of Products

  • 生物活性

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  • 参考文献

描述

15-PGDH/HPGD Protein, Human (HEK 293, His) is a recombinant human 15-PGDH/HPGD Protein expressed in HEK293 with a His tag at the N-terminus. 15-PGDH/HPGD, a key enzyme in the degradation of prostaglandins, plays an important role in the development of inflammation-related diseases[1].
研究背景

15-PGDH/HPGD是前列腺素降解的关键酶，包括前列腺素E2（PGE2），可以催化PGE2的15-羟基转化为15-酮基，产生无生物活性的PG，拮抗前列腺素的功能。前列腺素合酶 COX-2[1]。
生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
种属

Human
表达系统

HEK293
标签

C-6*His
蛋白编号

P15428 (M1-Q266)
基因 ID
蛋白结构
N-term
15-PGDH (M1-Q266)
Accession # P15428
6*His
C-term
同用名
rHu15-hydroxyprostaglandin dehydrogenase [NAD(+)]/HPGD, His; 15-Hydroxyprostaglandin Dehydrogenase [NAD(+)]; 15-PGDH; Prostaglandin Dehydrogenase 1; HPGD; PGDH1
氨基酸序列

MHVNGKVALVTGAAQGIGRAFAEALLLKGAKVALVDWNLEAGVQCKAALDEQFEPQKTLFIQCDVADQQQLRDTFRKVVDHFGRLDILVNNAGVNNEKNWEKTLQINLVSVISGTYLGLDYMSKQNGGEGGIIINMSSLAGLMPVAQQPVYCASKHGIVGFTRSAALAANLMNSGVRLNAICPGFVNTAILESIEKEENMGQYIEYKDHIKDMIKYYGILDPPLIANGLITLIEDDALNGAIMKITTSKGIHFQDYDTTPFQAKTQ

分子量

Approximately 30.0 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
性状

溶液.
组分

Supplied as a 0.2 μm filtered solution of 20 mM HEPES, 150 mM NaCl, pH 7.4.
内毒素含量

<1 EU/μg, determined by LAL method.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (209 KB)

产品使用指南 (1213 KB)
参考文献

15-PGDH/HPGD Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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15-PGDH/HPGD Protein, Human (HEK293, His)
目录号：
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