ACOX1 蛋白, Human (sf9, His)

The ACOX1 protein is critical in the initial steps of peroxisomal β-oxidation, especially of very long-chain fatty acids. It catalyzes the desaturation of fatty acyl-CoA (such as palmitoyl-CoA) to 2-trans-enoyl-CoA. ACOX1 Protein, Human (sf9, His) is the recombinant human-derived ACOX1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of ACOX1 Protein, Human (sf9, His) is 660 a.a., with molecular weight of ~60 kDa.

ACOX1 蛋白在过氧化物酶体 β-氧化的初始和限速步骤中发挥着关键作用，特别是直链饱和和不饱和极长链脂肪酸。其催化活性涉及将脂肪酰辅酶A（例如棕榈酰辅酶A）去饱和成2-反式烯酰辅酶A，例如（2E）-十六烯酰辅酶A。此外，ACOX1 通过直接向分子氧 (O(2)) 提供电子来促进过氧化氢 (H(2)O(2)) 的产生。虽然对中链脂肪酰辅酶 A 表现出显着的活性，但在 10 个碳的链长下观察到其最佳活性，以癸酰辅酶 A 为例，如体外所证明的。这种多方面的酶活性强调了 ACOX1 在过氧化物酶体 β-氧化和各种脂肪酸底物代谢中的不可或缺的作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

AAH08767.1 (M1-L660)

51  [NCBI]

Approximately 60 kDa

Greater than 85% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.0, 20% gly, 3 mM DTT.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.