ALKAL1 蛋白, Human (His, B2M)

ALKAL1 Protein, Human (His, B2M) is a potent activating ligand for human ALK that binds to the extracellular domain of ALK.

ALK 和 LTK 配体 1 (ALKAL1) 也称为“augmentor-β”或“FAM150A”，被确定为人类 ALK 的有效激活配体，可与 ALK 的胞外结构域结合。ALKAL1 在结直肠癌组织和细胞系中表达上调。ALKAL1 的上调与结直肠癌的肿瘤恶性程度和不良预后相关。ALKAL1沉默抑制结直肠癌细胞的肿瘤发生、转移和侵袭，并抑制SHH信号通路，这对于ALKAL1诱导的迁移至关重要。这些研究结果揭示了 ALKAL1 通过激活 SHH 信号通路参与结直肠癌迁移和侵袭的新机制^[1]。

Human

E. coli

N-6*His;N-B2M

Q6UXT8 (28R-129T)

389658  [NCBI]

RPRGRRGARVTDKEPKPLLFLPAAGAGRTPSGSRSAEIFPRDSNLKDKFIKHFTGPVTFSPECSKHFHRLYYNTRECSTPAYYKRCARLLTRLAVSPLCSQT

Approximately 30 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. V Van Deuren, et al. Structural determinants of nucleobase modification recognition in the AlkB family of dioxygenases. DNA Repair (Amst). 2020 Dec;96:102995.  [Content Brief]

  [2]. B J Chen, et al. The Escherichia coli AlkB protein protects human cells against alkylation-induced toxicity. J Bacteriol. 1994 Oct;176(20):6255-61.  [Content Brief]