ANGPTL3/Angiopoietin-like 3 蛋白, Human (204a.a, HEK293, His)

ANGPTL3/Angiopoietin-like 3 Protein, Human (204a.a, HEK293, His) plays an important role in lipoprotein metabolism. Angiopoietin-like Protein 3 acts as a dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL). Angiopoietin-like Protein 3 inhibits endothelial lipase hydrolysis of HDL-phospholipid (PL), thereby increasing HDL-PL levels.

血管生成素样蛋白 (ANGPTL) 是一个由八种分泌糖蛋白组成的家族，它们与血管生成素表现出结构同源性，并具有不同的生理功能，包括在脂质代谢、干细胞扩增、炎症、组织重塑和血管生成中的假定作用。近年来，三种ANGPTL，ANGPTL3、ANGPTL4和ANGP-TL8，已被证明在脂质代谢和血脂水平调节中发挥作用。小鼠和人类中 ANGPTL3 缺陷的发现激发了一系列研究，阐明了 ANGPTL3 在脂蛋白代谢中的作用。这些研究表明 ANGPTL3 可能是治疗人类血脂异常的新治疗靶点。最近旨在抑制 ANGPTL3 的干预试验的结果似乎支持这一假设。

Immobilized Human at ANGPTL3 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human ANGPTL3 Antibody, the ED₅₀ of human ANGPTL3 protein is 2.741 ng/mL, corresponding to a specific activity is 3.6483×10^5 units/mg.

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  Immobilized Human at ANGPTL3 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human ANGPTL3 Antibody, the ED₅₀ of human ANGPTL3 protein is 2.741 ng/mL, corresponding to a specific activity is 3.6483×10⁵ units/mg.

Human

HEK293

C-6*His

Q9Y5C1 (S17-P220)

27329  [NCBI]

SRIDQDNSSFDSLSPEPKSRFAMLDDVKILANGLLQLGHGLKDFVHKTKGQINDIFQKLNIFDQSFYDLSLQTSEIKEEEKELRRTTYKLQVKNEEVKNMSLELNSKLESLLEEKILLQQKVKYLEEQLTNLIQNQPETPEHPEVTSLKTFVEKQDNSIKDLLQTVEDQYKQLNQQHSQIKEIENQLRRTSIQEPTEISLSSKPHHHHHH

Approximately 35.44 kDa

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  Greater than 95 % as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Tarugi P, et al. Angiopoietin-like protein 3 (ANGPTL3) deficiency and familial combined hypolipidemia. J Biomed Res. 2019 Apr 22;33(2):73-81.  [Content Brief]

  [2]. Zeng L, et al. Identification of a novel human angiopoietin-like gene expressed mainly in heart. J Hum Genet. 2003;48(3):159-62.  [Content Brief]