Animal-Free IFN-gamma 蛋白, Mouse (His)

IFN-gamma is a type II interferon family cytokine that participates in antiviral, antibacterial, and antitumor responses. Animal-Free IFN-gamma Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIFN-gamma protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IFN-gamma Protein, Mouse (His) is 133 a.a., with molecular weight of ~16.5 kDa.

IFN-gamma 由免疫细胞如 t 细胞和 NK 细胞产生，通过激活效应免疫细胞和增强抗原呈递，在抗菌、抗病毒和抗肿瘤反应中起关键作用^[1][2][5][6]。FN-gamma 通过影响造血干细胞的发育、静止和分化，参与造血干细胞在发育和稳态条件下的调控^[3][4]。IFN-gamma 通过调节 Fas/FasL、TNF-相关凋亡-诱导配体 (TRAIL)、caspase-8、-3、-7 和 -1、survivin和 Bim 的表达来增加癌细胞对外在和内在凋亡途径的易感性^[5]。IFN-gamma 主要通过 JAK-STAT 通路与其受体 IFNGR1 相互作用，影响基因调控。IFN-gamma 与受体结合后，IFNGR1 的胞内结构域打开，允许下游信号元件 JAK2、JAK1 和 STAT1 结合，导致 STAT1 激活、核易位和 IFN-gamma 调控基因转录^[6]。IFN-gamma 通过诱导RNA活化蛋白激酶R (PKR)和腺苷脱氨酶RNA特异性-1 (ADAR-1)来实现抗病毒作用^[6]。IFN-gamma 能抑制 TH1 细胞产生 IL-4 并且维持 T-bet 的持续表达^[7]。IFN-gamma 作为细胞介导的免疫中心效应因子，可以通过同源t细胞相互作用增强抗原识别，通过抗原呈递细胞 (APCs) 放大抗原呈递，增加活性氧 (ROS) 和活性氮中间体 (RNIs) 的产生，诱导抗病毒反应^[8]。

Measure by its ability to anti-viral assay in L-929 cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <0.5 ng/mL. The specific activity of recombinant mouse IFN gamma is approximately >2x10⁶ IU/mg

Mouse

E. coli

C-His

P01580 (H23-C155)

15978  [NCBI]

MHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQIISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQLLPESSLRKRKRSRC

Approximately 16.5 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.01 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.